HYPOTHESIS Lyophilized plasma (LP) is as safe and effective as fresh frozen plasma (FFP) for resuscitation after severe trauma. DESIGN Multicenter animal study. SETTING Animal laboratories, 2 level I trauma centers. PARTICIPANTS Thirty-two Yorkshire crossbred swine. INTERVENTIONS Lyophilized plasma was analyzed for factor levels and clotting activity before lyophilization and after reco...

A project was undertaken to develop mussel reference materials that were certified for their mass fractions of saxitoxin and decarbamoyl-saxitoxin. Fifteen laboratories from various European countries participated. Three of these had major responsibility for substantial parts of the work and overall coordination of the project. The project involved 4 main activities: (1) procurement and charact...

The present study describes how to process human postheparin plasma (PHP) containing hepatic triglyceride lipase (HTGL) that is utilized as a standard material of HTGL for the quantification of HTGL mass in human plasma. The optimal storage conditions for PHP were established by monitoring the stability of HTGL molecules in PHP as an antigen, which was stored in the liquid, frozen, or lyophiliz...

Commercial quality-control materials and frozen plasma and serum pools were evaluated for high-density lipoprotein (HDL) cholesterol quantitation by the heparin-Mn2+ and phosphotungstate-Mg2+ precipitation methods and by ultracentrifugal and electrophoretic methods. We evaluated among-day precision; inter-method differences in HDL cholesterol quantitation; lipid composition and electrophoretic ...

Many different materials are available for grafting during surgery for Peyronie's disease (PD). To study the outcomes associated with the use of a lyophilized bovine pericardium graft (Peri-Guard®) to repair tunical defects in patients with PD, descriptive study of 43 patients with disabling penile curvature was performed. Curvature was measured before and after the intervention using a standar...

Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. With the exception of Nostoc muscorium, none of the organisms survived lyophil...

Pettersson et a!. recently reported that prostate-specific antigen (PSA) corn-plexed to a1-antichymotrypsin (PSA-ACT) is unstable in aqueous solutions [1]. Recognizing the usefulness of PSA-ACT calibrators, given that most of the PSA in male serum is in the complexed form, they pointed out that establishment of optimal conditions for preparation and storage of PSA-AGT complexes to prevent disso...