Comparisons of lens fiber cell membrane isolation methods were made. Although membrane lipid yield from stirring overnight in hypotonic medium followed by low-speed centrifugation was comparable to that from homogenization and high-speed centrifugation, losses in phosphatidyl ethanolamine were observed with the former method. Lyophilization with vacuum replacement by nitrogen did not exert any ...

In this study we lyophilized three types of live attenuated polioviruses (Sabin vaccine strains) and evaluated the lyophilized vaccine viruses' heat stability. The virus titers were measured after heating at 37 and 45 and then compared with the titers of conventional liquid vaccine viruses similarly treated. The results showed that lyophilization, while slightly reducing vaccine virus titers,...

It has been established that the mouse mam mary tumor agent (MTA), with the properties of an infectious agent or virus, will withstand filtra tion, lyophilization, and desiccation (1, 2-4, 8-10, 15). Gye (11) and Mann (12-14) advanced the theory that the agent would be liberated in its active form following freezing and would induce mammary cancer within a short time, because of its "selective ...

Haemagglutination of bluetongue virus (BTV) was demonstrated for the first time by making use of a purified preparation of the virus. The reaction was found to be independent of variations in the pH, temperature, buffer system and origin of the erythrocytes used in the test. A haemagglutination inhibition test, subsequently developed, was demonstrated to be serotype specific. The storage of the...

Staphylococcus aureus phage ISP was lyophilized, using an Amsco-Finn Aqua GT4 freeze dryer, in the presence of six different stabilizers at different concentrations. Stability of the lyophilized phage at 4 °C was monitored up to 37 months and compared to stability in Luria Bertani broth and physiological saline at 4 °C. Sucrose and trehalose were shown to be the best stabilizing additives, caus...