The transformation of blood lymphocytes in cultures with phytohaemagglutinin was studied in five patients before and at intervals up to 18 to 19 months after therapeutic irradiation of the abdomen and pelvis (dose of x rays 3,200r to 4,475r). In one patient, all cultures showed satisfactory lymphocyte transformation. In four patients transformation was depressed following irradiation. In three ...

The transformation response of peripheral blood lymphocytes in vitro to human arterial and muscle antigen has been studied in patients with polymyalgia rheumatica, polymyositis, rheumatoid arthritis, and polyarteritis, and polyarteritis nodosa and in unrelated controls. Lymphocytes from patients with polymyalgia rheumatica showed transformation responses significantly higher to artery antigen t...

Interleukin 10 (I1.10) is a pleiotropic factor that enhances proliferation of activated human B lymphocytes and induces them to secrete high amounts of immunoglobulins. Here we show that several human B cell lines were able to constitutively secrete human (h)Ibl0. Whereas none of the pre-B nor the plasmocytic cell lines tested produced hi1-10, 25 of the 36 tested mature B cell lines (lymphoblas...

Viral antigen prepared by heat inactivation of vaccinia virus stimulated production of interferon in association with transformation of sensitized human lymphocytes in vitro. Involvement of a macrophage-lymphocyte interaction in production of interferon stimulated by viral antigen was found in which macrophage greatly augmented the amount of interferon produced by lymphocytes. Reimmunization wi...

Two neutral proteinases from human polymorphonuclear leukocytes (PMN), an elastase and the chymotrypsin-like cathepsin G, were purified, and their actions on lymphocytes in culture were studied. Both PMN proteinases stimulate lymphocytes from human peripheral blood and from mouse spleen in vitro, but do not affect thymic cells from either normal or hydrocortisone-treated mice. In stimulated mou...

Two mouse anti-human monoclonal antibodies (S3.13 and S5.7) raised against cells of acute myelogenous leukemia were found to react with antigens expressed on the surface of subsets of monocytes and lymphocytes. S3.13 precipitates a peptide of Mr 29,000, and S5.7 precipitates a peptide of Mr 20,000 present on the surface of all the cell types tested. These two surface antigens were distributed o...

Enzyme cytochemical and histochemical tests have practical value for the study of immature, blast cells and lymphocytes since Romanowsky-stained films might be insufficient to identify differentiation and maturation features of the cells [2, 7]. Peripheral blood lymphocyte (PBL) counts and their enzymatic profile may change in the human [7] and bovine leukemia [23], Marek’s disease in the chick...

The expression of receptors for interleukin-6 (IL-6) on human and rhesus monkey peripheral blood and bone marrow (BM) cells was examined by multiparameter flow cytometry after staining with biologically active, biotin-labeled human IL-6 and phycoerythrin-conjugated streptavidin. Consistent with the multiple biologic effects of IL-6 in stimulating immune functions and hematopoiesis, IL-6 recepto...

Therapy planning in patients with myelodysplastic syndromes (MDSs) is complicated by its high prognostic heterogeneity. Forty-one patient and disease characteristics at onset of 370 patients with MDS were analyzed to identify significant prognostic factors for survival and transformation to acute myeloblastic leukemia (AML), and to develop and validate a regression model for predicting survival...