We have developed a Quantitative Light Absorption Analysis (QLAA) method to rapidly estimate human lymphocyte concentrations isolated from small volumes of whole blood. Measurements of the light absorption analysis were calibrated for lymphocyte concentration levels using a hemocytometer. To validate the QLAA system, blood samples were collected from 17 healthy donors and lymphocyte absorption ...

The neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte (PLR), and eosinophil count have been used as biomarkers of immune-related adverse events (irAEs) associated with immune checkpoint inhibitor (ICI) treatment. As no report has yet compared by the affected organs, we explored this topic. A total 284 patients on ICIs included 102 irAEs 182 without. following comparisons were statistically...

In this study, it was aimed to investigate the protective effect of Allium schoenoprasum L. plant against acrylamide toxicity. As animal material, 32 Wistar-Albino female rats were divided into 4 groups. The first group formed control group. second group, 25 mg/kg Acrylamide administered by gastric gavage every day for 15 days. third 200 ethanol extract given fourth + a result analysis, althoug...

Background Central immunity components especially microglia in dementia have been well studied and corresponding immunotherapy gradually caught the attention. However, few studies focused on peripheral dementia. Method To address issue, we examined longitudinal association between incident markers encompassing immune cell counts, their derived ratios including neutrophil-to-lymphocyte ratio (NL...

The pathogenesis of diabetic retinopathy is still challenging, with recent evidence proving the key role inflammation in damage retinal neurovascular unit. This study aims to investigate predictive value neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), lymphocyte-to-monocyte (LMR), and systemic index (SII) for (DR) its severity. We performed a retrospective on 129 T2DM patien...

Background Recurrent spontaneous abortion (RSA) is a complication in pregnancy that results into fetus rejection by mother and several factors such as anatomical, genetic and immunological problems can be its etiologic causes. Paternal lymphocyte therapy as an immunotherapy for these patients has been more evaluated. The purpose of lymphocyte therapy is stimulation of the mother immune system t...

A method to determine low density lipoprotein (LDL) uptake of distinct lymphocyte subpopulations was developed using fluorescent LDL and subsequent staining of lymphocyte subsets with biotinylated monoclonal antibodies plus streptavidin-CyChrome. LDL uptake was detected on a single cell level and semiquantified by FACS analysis. This method allows comparison of defined lymphocyte subsets from d...