Fractionation processes of fish oil fatty acid for the production of long-chain polyunsaturated fatty acids (PUFA) of omega-3 series have become very important because of the beneficial properties of these substances to human health, and, as a functional food. To obtain high yield of extraction and concentration of omega-3, rainbow sardine fish (Dussumieria acuta) oil were studied by: supercrit...

We observed increasing unserotypable (UT) Actinobacillus pleuropneumoniae isolates using agar gel diffusion (AGD) test. To reanalyze their serovar, we performed rapid slide agglutination (RSA) test and multiplex PCR for 47 UT isolates. Of these, 25 were serovar 1 (UT-serovar 1), 20 were serovar 2 (UT-serovar 2) and 2 were serovar 15 (UT-serovar 15). We examined serotyping antigen extraction tem...

The goal of this study is to investigate the immunoregulative effects of Panax japonicus polysaccharide (PJPS) on mice of low immunity. An orthogonal experiment was designed to determine the best extraction process for PJPS. By the tests of macrophages swallow chicken red blood cells, Delayed-type hypersensitivity (DTH), and serum hemolysin value, we studied the immune adjustment ability of PJP...

chilling is an important environmental stress that influences the yield and quality of many agricultural crops. different plants use different systems to endure this stress and minimize its effects. one of these systems is enzymatic reaction. to find out more about responses of different grapevine species and cultivars to the low temperature conditions, their enzymatic changes were evaluated in...

Madhuca longifolia is a traditional medicinal plant used in the treatment of several diseases including cancer in Indian system of medicine. Even though the chemical composition, bioactive principles and medicinal value of M. longifolia leaf have investigated earlier, the extraction of phytochemical compounds is not yet optimized, because preparation of extract will be of great importance as it...

Effects of varying temperatures with constant pressure of solvent on extraction efficiency of two chemically different alkaloids were studied. Camptothecin (CPT) from stem of Nothapodytes nimmoniana (Grah.) Mabb. and piperine from the fruits of Piper nigrum L. were extracted using Accelerated Solvent Extractor (ASE). Three cycles of extraction for a particular sample cell at a given temperature...

Headspace solid-phase microextraction has been applied to the analysis of aroma compounds in vinegar. Silica fibre coated with Carboxen-polydimethylsiloxane was found to be more efficient at extracting these compounds than other fibres such as those coated with polydimethylsiloxane, Carbowax-divinylbenzene, and polydimethylsiloxane-divinylbenzene, but its repeatability was low. Different parame...

DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic when subjected to polymerase chain reaction, especially when mature tissues are used for DNA extraction. In order to overcome the problems associated with poor-quality DNA extracted from such plant samples, a protocol has been developed, availing on a high salt concentration and on the combination of polyv...

OBJECTIVES This work aimed to enhance anti-skin cancer activities of Apostichopus japonicus, spiky sea cucumber, through ultrasonification extraction process at low temperature. METHODS Dried Apostichopus japonicus was extracted with an ultrasonification process at 50°C and 95 kHz for two hours (UE), and anti-skin cancer activities of the extract from the UE were also compared with those from...

An efficient and environmental-friendly extraction method has been developed for extraction of ursolic acid (UA) from Hedyotis diffusa by using subcritical water extraction (SWE). The experiments were carried out at different particle sizes (20–100 mesh), extraction temperature (120–200 ◦C), extraction time (10–50 min), solvent/solid ratio (20–40 mL/g), and extraction pressure (0.6–3.0 MPa). Re...