Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as a method to obtain a feline lipoprotein profile....

BACKGROUND: Cholesterol ester transfer protein (CETP) plays a major role in regulating the levels of LDL- and HDL-cholesterol. We previously observed a fish-oil-induced elevation of low-density lipoprotein (LDL)-and very-low-density lipoprotein (VLDL)-cholesterol concentrations and a decrease in high-density lipoprotein (HDL)-cholesterol concentration in F1B hamsters. The molecular mechanism/s ...

The efficacy of phytosterols and phytostanols added to foods and food supplements to obtain significant non-pharmacologic serum and low density lipoprotein (LDL) cholesterol reduction is well documented. Irrespective of age, gender, ethnic background, body weight, background diet, or the cause of hypercholesterolemia and, even added to statin treatment, phytosterols and phytostanols at 2 g/day ...

In clinical practice, the coronary artery disease (CAD) risk associated with high levels of low-density lipoprotein (LDL) or low levels of high-density lipoprotein (HDL) is assessed not by measuring LDL and HDL particles directly, but by measuring the amount of cholesterol carried by these lipoproteins. It is not generally appreciated how much the amount of cholesterol per particle varies from ...

Introduction: Abnormal lipid profile causes maternal mortality and impairs fetal development. This study investigated the effect of Ramadan fasting on plasma lipid profile in pregnant women. Material and Methods: This self-control, cohort study was performed in healthy volunteer fasted pregnant women. Serum cholesterol, triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein ...

OBJECTIVE Progression of carotid intima-media thickness (CIMT) is a surrogate indicator for the early stages of atherosclerosis. METHODS The study investigated relationships between baseline lipoprotein cholesterol, triglyceride (TG), and apolipoprotein (Apo) B levels assessed with density gradient ultracentrifugation (DGU) and progression of posterior wall common CIMT in men (45-75 years of ...

Endotoxin induces hyperlipidaemia in experimental animals. In the current study, we investigated whether endotoxin alters hepatic low-density lipoprotein (LDL) receptor expression in rats. Endotoxin treatment suppressed hepatic LDL receptor expression in a dose- and time-dependent manner. Eighteen hours after intraperitoneal injection of increasing amounts of endotoxin, LDL receptor and its mRN...

By equilibrium density gradient ultracentrifugation, we analyzed the chemical composition and particle size of low density lipoproteins (LDLs) in 16 subfractions separated from the LDL fractions (1.019 less than d less than 1.063 g/ml) of two hyperalphalipoproteinemic patients who had a deficiency of cholesteryl ester transfer protein (CETP). The LDLs of these patients comprised a group of hete...

The 12th annual scientific meeting of the European Vascular Biology Association was held in Gilleleje, Denmark, from April 27 to May 1, 1991, in collaboration with the Scandinavian Society for Atherosclerosis Research. The sessions of the meeting covered two major fields: hyperlipidemias relating to the genetic variability of apolipoprotein B-100 and transendothelial flow of macromolecules. The...