The postomortem invasion of muscle and other tissues by bacteria from the intestinal tract was studied with the use of radioactive tracers. The injection of 14C-labeled bacteria or spores into the intestines of guinea pig carcasses within 24 h of death resulted in the rapid spread of 14C throughout carcasses. When live bacteria were injected along with the labeled cells, it was not possible to ...

We demonstrate a methodology for tracing the clonal history of hematopoietic stem and progenitor cells (HSPCs) behavior in live tissues in 4 dimensions (4D). This integrates genetic combinatorial marking using lentiviral vectors encoding various fluorescent proteins (FPs) with advanced imaging methods. Five FPs: Cerulean, EGFP, Venus, tdTomato, and mCherry were concurrently used to create a div...

Drought causes an increase in some gene expression in plant tissues such as plasma membrane intrinsic proteins type 1 (PIP1), 9-cis-epoxycarotenoid dioxygenase (NCED) SlAREB1. The effects of exogenous abscisic acid (ABA) and two sulfonamide compounds, namely, sulfacetamide (Sa) and sulfasalazine (SS) were studied on gene expression of tomato (Lycopersicon esculentum Mill. Cv. Super chief) under...

Endophytic fungi constitute a remarkable multifarious group of microorganisms live within plants tissues without causing any obvious negative effect. Endophytic fungi have been found in every plant species examined to date. During an investigation on endophytic fungi, 123 fungal isolates were obtained from healthy twigs and leaves of cherry (Prunus avium) and sour cherry (P. cerasus) trees in I...

Background: Infections include soft tissue infections are notable reason for hospital admission among IDUs, owing to unsterile injection techniques and equipment, contamination of drugs with organisms, and poor hygiene. In present case report a patient with profound limb infection is introduced. Case: A 32 years old man was transferred to the emergency department. He was IDU with Iranian for...

One of the key enabling features of coherent Raman scattering (CRS) techniques is the dramatically improved imaging speed over conventional vibrational imaging methods. It is this enhanced imaging acquisition rate that has guided the field of vibrational microscopy into the territory of real-time imaging of live tissues. In this feature article, we review several aspects of fast vibrational ima...

OBJECTIVE Metastasis-associated in colon cancer-1 (MACC1), a new gene associated with primary and metastatic colon cancer, promotes tumor cell growth as well as the development of distant metastasis. The aim of this study is to investigate the expression of MACC1 protein in colon cancer and its association with clinicopathological parameters and postoperative liver metastasis. MATERIALS AND M...

Two-photon excitation (TPE) probe-based fluorescence imaging has become one of the most attractive diagnostic techniques to investigate biomolecules and biological events in live cells and tissues. At the current stage most of the TPE-based sensing is reflected by fluorescence intensity changes. Nevertheless the mere altering of intensity could be facilely affected by ambient conditions. On the...

The capillary electrophoresis assay described in this study is reproducible, more sensitive and faster than other analytical tests. The samples used in the capillary electrophoresis assay were obtained from brain and the lymphoid system of the animals. The sensitivity of this assay made it possible to test samples from other tissues that contain much less abnormal prion protein than brain sampl...

Phosphorus Magnetic Resonance Spectroscopy (31P-MRS) has been utilized to study energy, carbohydrate, and phospholipid metabolism in vitro and in vivo in live tissues non-invasively. Despite its lack of sensitivity, its application has extended to in situ human tissues and organs since proper signal localization was devised. Follow-up of phosphocreatine in neuromuscular diseases and schizophren...