A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and dif...

Spleen cells of mice infected with Listeria monocytogenes were adoptively transferred to normal mice. Such lymphocytes conferred resistance to a lethal challenge with Listeria. Hyperimmunization of the donor reduces the number of cells necessary to transfer effective immunity. Such spleen cells if treated with anti-theta serum do not transfer resistance to Listeria. Hence, thymus (T) lymphocyte...

Background Listeria sp. are facultative intracellular bacterial pathogens, which pose a potential public health problem related to consumption of contaminated food. Human listeriosis is clinically classified as perinatal listeriosis, neonatal listeriosis and adult listeriosis. Human infections caused by Listeria sp. present typically as meningitis. Other infections attributed to Listeria sp. in...

Background: Listeria consists of both pathogenic and non-pathogenic species. Reports of similarities between the genomic content between some pathogenic and non-pathogenic species necessitates the investigation of these species at the genomic level to understand the evolution of virulence-associated genes. With Listeria genome data growing exponentially, comparative genomic analysis may give be...

We sequenced the genome of Listeria ivanovii strain G770, which caused a deadly infection of the thoracic aortic prosthesis of a 78-year-old man. The 2.9 Mb genome exhibited 21 specific genes among L. ivanovii strains, including five genes encoding a type I restriction modification system and one glycopeptide resistance gene.

Listeria monocytogenes (commonly called Listeria) named for Joseph Lister. Listeria monocytogenes, is the bacterium that causes the infection listeriosis. It is a facultative anaerobic bacterium, capable of graving and reproducing inside the host’s cells, and is one of the most virulent food-borne pathogens of clinical infections resulting in death. Listeriosis is the leading cause of death amo...

Intracellular bacterial pathogens manipulate host cell functions by producing enzymes that stimulate or antagonize signal transduction. The Listeria monocytogenes genome contains a gene, lmo1800, encoding a protein with a conserved motif of conventional tyrosine phosphatases. Here, we report that the lmo1800-encoded protein LipA is secreted by Listeria and displays tyrosine as well as lipid pho...

Background: Food preservation includes procedures to assure higher level of food safety, hygiene and stability. To achieve this goal, the use of combination biopreservatives, such as Essential oils and Probiotics, has gained increased attention. Objective: The aim of this study was to evaluate the antimicrobial effects of Mentha longifolia L. esential oil and Lactobacillus casei aginst Listeri...

background the genus-listeria has two pathogenic species namely, listeria monocytogenes and listeria ivanovii. of these, l. monocytogenes is a well-known cause of abortion, encephalitis and septicaemia in man and animals. listeria ivanovii is an animal pathogen and in rare cases causes human infection. contamination of ovine carcasses during the slaughter and processing is a major risk for food...

A total of 336 Listeria isolates from ready-to-eat (RTE) meat products and meat-processing environments, consisting of 206 Listeria monocytogenes, and 130 Listeria innocua isolates, were characterized by disc diffusion assay and minimum inhibitory concentration (MIC) values for antimicrobial susceptibility against twenty antimicrobials. Resistance to one or two antimicrobials was observed in 71...