نتایج جستجو برای: linker b lentivirus plasmid after confirmation of cloning
تعداد نتایج: 21379645 فیلتر نتایج به سال:
Background and Objective: Toxoplasmosis, caused by an intracellular protozoan parasite, Toxoplasma gondii, is widespread throughout the world. Being a cause of congenital disease and abortion in humans and in domestic animals, the disease is of major medical and veterinary importance. In addition, it has increased importance due to toxoplasma encephalitis in AIDS patients. Therefore, developi...
abstract: proverbs are of the language materials which of the food of any language depends on the quality and quantity of these same materials. in the present research, the researcher tried to present the ramsarian proverbs according to four variables of color, fruit, plantsand animals that selected from the book of the members of each of these four variables, put them into the spss system fo...
Background and Aims: Newcastle disease virus (NDV) is one of the major pathogen in poultry. Vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. F gene open reading frame (ORF) from NDV is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. The F glycoprotein on the surf...
this research was conducted to examine the effect of a concurrent training on rest level of leptin of plasma and some hormonal factors in non-athlete subjects. the research population included non-athlete men who didn’t participate in any organized sport activities. 30 men were chosen voluntarily among the above-mentioned population and were divided into 2 groups: experimental (15 subjects) an...
Introduction Escherichia coli has a direct relationship with the diarrheagenic diseases, which occur quite in developing countries due to poor sanitation system and public health. It mainly affects children, causing a rate of morbidity and mortality worrying. This bacterium has a versatility of strains expressing different mechanisms and virulence factors, thus the importance in studying the ta...
Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...
Objective: Inhibition of IL-17 on periodontal ligament stem cells (PDLSCs) proliferation osteogenic differentiation had been reported. This study was to investigate the effect of IL-17 on human PDLSCs (hPDLSCs). Methods: Isolated hPDLSCs from healthy fresh premolars were identified and then transfected with IL-17 silencing lentivirus plasmid. Then cell proliferation, colony formation, apoptosis...
The FliG protein is a central component of the bacterial flagellar motor. It is one of the first proteins added during assembly of the flagellar basal body, and there are 26 copies per motor. FliG interacts directly with the Mot protein complex of the stator to generate torque, and it is a crucial player in switching the direction of flagellar rotation from clockwise (CW) to counterclockwise an...
Shuttle vectors (pMS3 and pMS4) which replicated in Escherichia coli and in gram-positive Acetobacterium woodii were constructed by ligating the replication origin of plasmid pAMbeta1 with the E. coli cloning vector pUC19 and the tetM gene of streptococcal transposon Tn916. Electrotransformation of A. woodii was achieved at frequencies of 4.5 x 10 transformants per mug of plasmid DNA. For conju...
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