rho-kinase is an effector molecule of rhoa, a monomeric gtp-binding protein, and causes ca2+ sensitization through inactivation of myosin phosphatase. the major physiological functions of rho/rho-kinase cascade include contraction, proliferation and migration in cells.there are some excellent reviews about rho/rho-kinase signal pathway, most of which focus on the specific proteins of the pathwa...

Pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and ADP to yield ATP and Pyruvate. Geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. Given that limited proteolysis experiments can be successfully used to probe conformational features of p...

Our laboratory has shown that dopamine D(2)-like receptor activation causes stimulation of Na(+), K(+)-ATPase (NKA) activity in the proximal tubules of the rat kidney. The present study was designed to investigate the cellular signaling mechanisms mediating this response to D(2)-like receptor activation. We measured the stimulation of NKA activity by bromocriptine (D(2)-like receptor agonist) i...