A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr(-1) mg(-1) fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acet...

The relatively high level of fatty acids in soybean nodules and rhizobia from soybean nodules suggested that the glyoxylate cycle might have a role in nodule metabolism. Several species of rhizobia in pure culture were found to have malate synthetase activity when grown on a number of different carbon sources. Significant isocitrate lyase activity was induced when oleate, which presumably may a...

Objective: Drought stress is most important factor which reduce root and shoot growth in crops. This study was performed in order to evaluate the effects of drought stress and Zn fertilizer on root traits of four cultivars of chickpea. Methods: Experiment was laid out in a split plot-factorial using randomized complete block design with three replications. Drought stress treatment stand in main...

Nitric oxide (NO) is emerging as an important regulatory player in the Rhizobium-legume symbiosis, but its biological role in nodule functioning is still far from being understood. To unravel the signal transduction cascade and ultimately NO function, it is necessary to identify its molecular targets. This study provides evidence that glutamine synthetase (GS), a key enzyme for root nodule meta...

This paper integrates competitive pricing and network design problems for the short life cycle products. The pricing problem determines selling prices of the products for different life cycle phases in a competitive market, as well as acquisition management of returned products. Besides, the selling and acquisition prices are related to the distance between distribution centers and customers. T...