نتایج جستجو برای: lentiviral

تعداد نتایج: 5695  

Journal: :Science 2002
Carlos Lois Elizabeth J Hong Shirley Pease Eric J Brown David Baltimore

Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescenc...

Journal: :Immunopharmacology and Immunotoxicology 2010

2015
Yingying Mao Renhe Yan Andrew Li Yanling Zhang Jinlong Li Hongyan Du Baihong Chen Wenjin Wei Yi Zhang Colin Sumners Haifa Zheng Hongwei Li

OBJECTIVES Lentiviral vectors have been used successfully to rapidly produce decigram quantities of active recombinant proteins in mammalian cell lines. To optimize the protein production platform, the roles of Ubiquitous Chromatin Opening Element (UCOE), an insulator, and selected promoters were evaluated based on efficiency and stability of foreign gene expression mediated by lentiviral vecto...

Journal: :research in pharmaceutical sciences 0

lentiviral vectors are among the promising viral based-vectors in gene therapy applications, but the efficiency of their targeting needs to be improved. (strept)avidin-biotin adaptor system is a novel approach to modify the lentiviral envelope for better targeting properties. herein, we describe utilization of this adaptor system by designing a candidate envelope protein-bearing biotin acceptor...

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