BACKGROUND This study aimed to define the occurrence of different organisms causing vulvovaginitis; to evaluate different laboratory methods used for diagnosis of Trichomonas vaginalis (T. vaginalis); and to evaluate the direct score system and clue cell method compared with culture for diagnosis of bacterial and T. vaginalis vaginosis. METHODOLOGY Clinical and laboratory evaluations were per...

Seven stains were studied to determine the best color and contrast for staining the developmental stages of free living pathogenic Acanthamoeba and Naegleria species. The acid-fast bacilli stain (AFB) produced a blue color without contrast; trichrome-eosin and modified Field's showed various color contrasts; Giemsa, iron-hematoxylin, modified AFB and Gram produced only one color which distingui...

Low parasitemic condition in malaria remains a diagnostic challenge; as the available diagnostic methods failed to detect. Currently, hemozoin (Hz) pigment is gaining attention in the diagnosis of malaria. The major drawback is ease of detection of Hz in routine practice. A pilot study was conducted to evaluate the role of Hz pigment and to compare the performance of quantitative buffy coat ass...

BACKGROUND The best sites for biopsy-based tests to evaluate H. pylori infection in gastritis with atrophy are not well known. This study aimed to evaluate the site and sensitivity of biopsy-based tests in terms of degree of gastritis with atrophy. METHODS One hundred and sixty-four (164) uninvestigated dyspepsia patients were enrolled. Biopsy-based tests (i.e., culture, histology Giemsa stai...

Objective: In our study, we aimed to evaluate the prevalence of Helicobacter pylori (H. Pylori) and characteristics antrum pathologies in patients with portal hypertensive gastropathy. Materials Methods: Antrum presence H. Pylori evaluated Giemsa stain gastroscopies 203 Results: A total patients, 119 male, 84 female, gastropathy was included study. hypertension, rate positivity 15.3%. This dete...

I N THE COURSE of cunrrent investigations on leukocyte granules, the questioni arose as to w’hether the group responsible for the stable sudanophilia might be a primary amine. It is well known that primary amines are differentially destroyed by nitrous acid,’3 thus: RNH2 + HNO2-ROH + H2O + N2. In testing this hypothesis we used both Vans Slyke’s reagent* arid molar solutionis of sodium nitrite ...

It is well established that a proportion of patients with tobacco amblyopia have pernicious anaemia or some other demonstrable defect of vitamin B12 metabolism (Leishman, 195I; Heaton, McCormick, and Freeman, I958; Freeman and Heaton, I96I; Foulds, Chisholm, Bronte-Stewart, and Wilson, I968). We have examined 65 cases of tobacco amblyopia diagnosed according to the criteria of Heaton and others...

Silicon (Si) is a beneficial, if not essential, plant nutrient (Epstein, 1994). As the second most abundant element in the Earth’s crust it has a global cycling budget similar to that of carbon (Conley, 2002). Some ecological roles of Si are characterized (Cooke and Leishman, 2011), but recent technological advances mean unprecedented understanding of functions at multiple scales, and recogniti...

Various in situ hybridization (ISH) methods have been used to identify Helicobacter pylori, a causative organism responsible for chronic gastritis and peptic ulcer disease, but they were hard to perform and time consuming. To detect H. pylori in a rapid and easily reproducible way, we developed synthetic biotinylated oligonucleotide probes which complement rRNA of H. pylori. Formalin-fixed and ...