Selective isolation of Kunitz trypsin inhibitor (KTI) and lectin from soybean whey solutions by different types of chitosan beads was investigated. The chitosan beads were co-crosslinked with tripolyphosphate/genipin in solutions at pH 5, 7 or 9 (CB5, CB7, CB9). The maximum adsorption ratios of chitosan beads to KTI and lectin were observed at pH 4.4 and 5.4, respectively; highly selective sepa...

Drosophila melanogaster has long been used as a model for the molecular genetics of innate immunity. Such work has uncovered several immune receptors that recognize bacterial and fungal pathogens by binding unique components of their cell walls and membranes. Drosophila also act as hosts to metazoan pathogens such as parasitic wasps, which can infect a majority of individuals in natural populat...

Crude extracts from Salvia sclarea seeds were known to contain a lectin which specifically agglutinates Tn erythrocytes (Bird, G. W. G., and Wingham, G. (1974) Vox Sang. 26, 163-166). We have purified the lectin to homogeneity by ion-exchange chromatography and affinity chromatography. The agglutinin was found to be a glycoprotein of Mr = 50,000, composed of two identical subunits of Mr = 35,00...

Entamoeba histolytica infection results in either asymptomatic colonization or invasive colitis and liver abscess. E. histolytica isolates from patients with invasive disease have characteristic isoenzyme profiles (pathogenic zymodemes), suggesting a role for parasite factors in determining the severity of infection. A galactose-specific cell surface lectin from a pathogenic zymodeme was shown ...

Background Lectins are highly important biomolecules to study several biological processes. A novel α-D-glucose/mannose specific lectin was isolated from the seeds of litchi fruits (Litchi chinensis) and its various biophysical and biochemical properties were studied. Methods Purification was done by successive Sephadex G 100 and Con A-Sepharose 4B affinity chromatography. SDS-PAGE, Surface P...

Viscum album and its endophytic fungal species lectin are reported as antioxidant, antimicrobial, anti-inflammatory and anticancer activity. The present investigation was undertaken to in vitro (by enzymes inhibition) and in vivo antidiabetic activity of endopytic fungi, Alternaria species N-acetyl-galactosamine, 54 kDa protein lectin in alloxan induced diabetes in rats. Endophytic fungal lecti...

This study evaluated the nutritional value of raw lectin-free soybeans in comparison with raw Kunitz trypsin inhibitor-free soybeans, raw conventional soybeans, and commercial heat processed soybean meal (SBM). Analyzed lectin values (milligrams per kilogram) were 7.2, 7.1, and < 0.00015 for the Kunitz-free, conventional, and lectin-free soybeans, respectively. Three experiments were conducted ...

Purpose: The mannose-binding lectin pathway of innate immunity is part of the first line of defense against microorganisms. Variations in mannose-binding lectin levels or activity are caused by single-nucleotide polymorphisms in the promoter region. Mannosebinding lectin may play a role as a phase reactant due to inflammatory processes related to cancer disease. Such inflammatory processes are ...

Reperfusion of ischemic tissues elicits an acute inflammatory response involving serum complement, which is activated by circulating natural IgM specific to self-Ags exposed by ischemia. Recent reports demonstrating a role for the lectin pathway raise a question regarding the initial events in complement activation. To dissect the individual roles of natural IgM and lectin in activation of comp...

Using an aMnity adsorbent prepared from L-fucose and starch, a lectin was isolated from fruit bedies of an ascomycete mushroom, IVdlelastiza ch teri. The lectin was found to cross-react with antiserum against Aleuria aurantia lectin (AAL), that had been obtained £ rom another ascomycete mushroom. The N-termina] amino acid sequence was analyzed, and among 20 residues 12 were the same as AAL. The...