BACKGROUND African swine fever (ASF) is a viral infectious disease of domestic and wild suids of all breeds and ages, causing a wide range of hemorrhagic syndromes and frequently characterized by high mortality. The disease is endemic in Sub-Saharan Africa and Sardinia. Since 2007, it has also been present in different countries of Eastern Europe, where control measures have not been effective ...

BACKGROUND Leptospirosis is a re-emerging infectious disease that is under-recognized due to low-sensitivity and cumbersome serological tests. MAT is the gold standard test and it is the only serogroup specific test used till date. Rapid reliable alternative serogroup specific tests are needed for surveillance studies to identify locally circulating serogroups in the study area. METHODS/PRINC...

OBJECTIVE The detection of urinary lipoarabinomannan (LAM), a mycobacterial cell wall component, is used to diagnose tuberculosis (TB). How LAM enters the urine is not known. To investigate if urinary LAM-positivity is the result of renal TB infection we correlated the outcomes of urinary LAM-antigen testing to renal histology in an autopsy cohort of hospitalized, Ugandan, HIV-infected adults. ...

We validate the use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection anti-SARS-CoV-2 IgM IgG in serum, plasma, whole blood, compare results with ELISA. also seek to establish value LFI testing on blood obtained from capillary sample. Samples collected by venous draw finger stick were patients SARS-CoV-2 detected RT-qPCR control patients. tested Biolidic...

Point-of Care (POC) devices that can perform multi-step processes, are easy to use, and are inexpensive, are needed for use in limited-resource settings. We present an integrated paper-based device that performs an automated immunoassay for the detection of IgM antibodies with on-board sample processing. The device is a two-dimensional paper network (2DPN) consisting of protein-G coated nitroce...

A rapid enzyme-linked immunosorbent assay (ELISA) test (microwell plate) and a membrane-based colloidal gold immunoassay in flow-through and lateral-flow formats for the rapid detection of fumonisin B1 (FB1) were developed. The rapid microwell assay can be completed within 20 min with the detection limit of 0.5 +/- 0.2 microg/L. Membrane-based colloidal gold immunoassays had a visual detection ...