A β-galactosidase from Cicer arietinum seeds has been purified to apparent electrophoretic homogeneity using a combination of various fractionation and chromatographic techniques, giving a final specific activity of 220 units mg(-1), with approximately 1840 fold purification. Analysis of the protein by SDS-PAGE revealed two subunits with molecular masses of 48 and 38 kDa, respectively. These ba...

protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in saccharomyces cerevisiae and kluyveromyces marxianus. crude protein content of dried yeast cells were 53.22% and 45.6% for s.cerevisiae and k.marxianus, respectively. after 96 hrs of...

The formation of galactooligosaccharides (GOS) in skim milk during treatment with several commercial β-galactosidases (Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae) was analysed in detail, at 4 and 40°C. The maximum GOS concentration was obtained at a lactose conversion of approximately 40-50% with B. circulans and A. oryzae β-galactosidases, and at 95% lactose depletion for ...

Digestion of lactose, the milk disaccharide, makes problem to a large number of the population. Therefore, the lactose intolerant individuals are limited in the intake of milk and dairy products. The lactose – free products offers a solution for the diet. Raman spectroscopic analyses were performed for a purpose of rapid assessment of lactose content in bovine milk, lactose-free milk, mixtures ...

The beta-galactosidase from the Antarctic gram-negative bacterium Pseudoalteromonas haloplanktis TAE 79 was purified to homogeneity. The nucleotide sequence and the NH(2)-terminal amino acid sequence of the purified enzyme indicate that the beta-galactosidase subunit is composed of 1,038 amino acids with a calculated M(r) of 118,068. This beta-galactosidase shares structural properties with Esc...

Nuclear magnetic resonance (NMR) spectroscopy was used to investigate the transfer of sialic acid from a range of sialic acid donor compounds to acceptor molecules, catalyzed by Trypanosoma cruzi trans-sialidase (TcTS). We demonstrate here that NMR spectroscopy is a powerful tool to monitor the trans-sialidase enzyme reaction for a variety of donor and acceptor molecules. The hydrolysis or tran...

This study aimed to evaluate the effect of Lacticaseibacillus casei 01 as a probiotic culture on production volatile organic compounds and metabolic profile Minas Frescal cheese. Lactose (α-lactose β-lactose), fatty acids (unsaturated saturated), citric acid, tryptophan, benzoic acid were main compounds. Compared with control cheese, cheese was characterized by highest concentration tryptophan ...

In this work, enzymatically active polysaccharide-based hydrogels and aerogels have been developed. To end, a thermostable ?-galactosidase (TmLac) enzyme from Thermotoga maritima embedded in nanoflowers’ format was used to evaluate the capacity of hydrogel matrices preserve hydrolytic activity reusability formed. Commercial agar, unpurified agar agarose were compared as supporting materials. Al...