LL-H, a virulent phage of Lactobacillus delbrueckii subsp. lactis, produces a peptidoglycan-degrading enzyme, Mur, that is effective on L. delbrueckii, Lactobacillus acidophilus, Lactobacillus helveticus, and Pediococcus damnosus cell walls. In this study, the LL-H gene mur was cloned into Escherichia coli, its nucleotide sequence was determined, and the enzyme produced in E. coli was purified ...

An aminopeptidase with a very broad substrate specificity was purified to homogeneity from Lactobacillus helveticus SBT 2171 by FPLC. The enzyme was purified 144-fold from a cell-free extract with a yield of 16%. The purified enzyme appeared as a single band on an SDS-PAGE gel. It had a molecular mass of 95 kDa and an isoelectric point of 4.9. The enzyme hydrolysed a large range of naphthylamid...

The bacterial communities of Zaopocu (ZPC), a tropical specialty fermented food in Hainan Island, were characterized this study using high-throughput sequencing. Firmicutes and Proteobacteria the two dominant phyla. primary genus ZPC_A was Weissella, while ZPC_B ZPC_C had Lactobacillus as genus. biomarker lactic acid bacteria (LAB) brevis, Weissella confusa, Leuconostoc citreum, whereas amyloly...

The profile of polyunsaturated fatty acids in cheeses obtained through fermentation by lactic acid bacteria Lactobacillus helveticus and Streptococcus thermophilus were evaluated. milk used to make the came from cows fed with flaxseed oil annato. presented microbiological physic-chemical quality standards established legislation for Staphylococci Listeria. With maturation, there was a reduction...

Lactobacillus helveticus DLBSA201 and DLBSA202 were investigated for their potential probiotic traits protective effects against Escherichia coli O157:H7. The survival rate of after being exposed to acid bile salt was relatively high, although no hydrolase activity detected. Both strains also demonstrated an outstanding ability adhere intestinal epithelial cells. A combination could interfere w...

Lactobacillus helveticus BGRA43 is a human intestinal isolate showing antimicrobial activity, amongst others, against Yersinia enterocolitica, Shigella sonnei, Shigella flexneri, and Streptococcus pneumoniae. BGRA43 produces PrtH proteinase with proteolytic activity on both casein and β-lactoglobulin (BLG). BGRA43 is able to reduce the allergenicity of BLG. Bioactive peptides released in BGRA43...

A pH-controlled continuous lactic acid fermentation of whey permeate supplemented with yeast extract was carried out with Lactobacillus helveticus entrapped in K-carrageenan-locust bean gum gel beads at various dilution rates and pH set points. A kinetic study of the immobilized cell continuous fermentation in a 1-I stirred-tank reactor containing 33% (v/v) gel beads was carried out with an ori...