BACKGROUND The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied CRISPR/Cas9 for the knock-in of 8-11 kb inserts into Rosa26 of C57BL/6 zygot...

Phosphorylation of mouse p53 at Ser18 occurs after DNA damage. To determine the physiological roles of this phosphorylation event in p53-dependent DNA damage responses, a Ser18 to Ala missense mutation was introduced into the germline of mice. Thymocytes and fibroblasts from the knock-in mice show reduced transactivation of many p53 target genes following DNA damage. p53 protein stabilization a...

The core of many workflow processes in banks, insurance companies, governmental departments, and administrations of multinationals is formed by a set of tasks that are used to classify cases into two groups: accepted and rejected. Each of these Ž . tasks has two possible outcomes: OK or NOK i.e., Not OK . If for a specific case all tasks result in OK, the case is accepted, otherwise it is rejec...

OSCAR is a web platform for cluster and crossspecies analysis of microarray data. It provides a comprehensive but friendly environment to both users and algorithm developers. For users, OSCAR provides cluster tools for both single and multiple species data, together with interactive analysis features. For single species data, OSCAR currently provides Hierarchical Clustering, K-means, partition ...

Previous studies with peritoneal macrophages obtained from macrophage scavenger receptor class A (MSR-A) knock-out mice showed that the endocytic uptake of advanced glycation end products (AGE) by macrophages was mediated mainly by MSR-A. However, it is controversial whether the endocytic uptake of intravenously injected AGE proteins by liver sinusoidal endothelial cells (LECs) is similarly exp...

Engine knock is an undesired phenomenon in spark ignited internal combustion engines; such as a gasoline engine running the Otto cycle. Knocking originates from abnormal combustions at timings other than those decided by controlled ignition. Individual abnormal combustions of this type is also referred to as auto-ignition. Knocking is not only a limitation on engine efficiency and thus of envir...

The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual cells, effectively resulting in knock-out of targeted genes. It thus promises to be an ideal candidate for application in neuroscience where constitutive genetic modificatio...

of Detonation (Knock) Using a Wavelet to Create a Chart for a Petrol Engine’s Characteristics Agus Sujono , R. Soekrisno , Eka Firmansyah , Oyas Wahyunggoro #4 # Faculty of Engineering, Sebelas Maret University (UNS), PhD student of Electrical Engineering, Gadjah Mada University (UGM) Surakarta , Indonesia # Faculty of Engineering, UGM, Yogyakarta, Indonesia 1 [email protected] 4 oyas@ug...

Fluorescent proteins and epitope tags can reveal protein localization in cells animals, yet the large size of many hinders efficient genome targeting. Accordingly, studies have relied on characterizing overexpressed proteins, which might not recapitulate endogenous activities. Here, we present two strategies for higher throughput production reporters mice, focusing blastocyst model development....

This paper asks what sorts of ethical caution ought to attach increasingly data-driven approaches understanding the brain. is taken be an important question especially owing a likely near future neuromonitoring and neuromodulation devices with applications in psychiatry. The explores this by i) sketching concept ‘digital twin,’ ii) drawing schematic picture ‘brain datafication’ general, iii) de...