OBJECTIVES MUC7 12-mer (RKSYKCLHKRCR), a cationic peptide derived from human salivary MUC7 mucin, exhibits potent in vitro antifungal activity, as determined by killing assays in phosphate buffer. In this study we examined the MUC7 12-mer antifungal activity alone or in combination with other antifungal agents in LYM medium (modified RPMI 1640). METHODS Antifungal activities of MUC7 12-mer an...

In this study we present a model of in vitro cell killing through type II Photodynamic Therapy (PDT) for simulation of the molecular interactions leading to cell death in time domain in the presence of oxygen transport within a spherical cell. By coupling the molecular kinetics to cell killing, we develop a modeling method of PDT cytotoxicity caused by singlet oxygen and obtain the cell surviva...

Granulosa cell tumors (GCT) constitute only ~5% of ovarian neoplasms yet have significant consequences, as up to 80% women with recurrent GCT will die the disease. This study investigated effectiveness procaspase-activating compound 1 (PAC-1), an activator procaspase-3, in treating adult (AGCT) combination selected apoptosis-inducing agents. Sensitivity AGCT line KGN these drugs, alone or PAC-1...

Despite illegal killing (poaching) being the major cause of death among large carnivores globally, little is known about effect implementing lethal management policies on poaching. Two opposing hypotheses have been proposed in literature: may decrease poaching incidence (killing for tolerance) or increase it (facilitated killing). Here, we report a test two opposed that (reported and unreported...

We have measured the frequency of UV-induced reversions (locus plus suppressor) for the ochre alleles ade2-1 and lys2-1 and forward mutations (ade2 adex double auxotrophs) in an excision-deficient strain of Saccharomyces cerevisiae (rad2-20). For very low UV doses, both mutational systems exhibit linear induction kinetics. However, as the dose increases, a strikingly different response is obser...

Double-strand breaks (DSBs) can be efficiently removed from the DNA of higher eukaryotes by nonhomologous end-joining (NHEJ). Genetic studies implicate the DNA-dependent protein kinase (DNA-PK) in NHEJ, but the exact function of this protein complex in the rejoining reaction remains to be elucidated. We compared rejoining of DNA DSBs in a human glioma cell line, M059-J, lacking the catalytic su...

Antigen-specific killing ability of effector CD8+ T cells is critical for protective immunity against infection. Here, we describe in vivo cytotoxic T cell assay to examine effector function of antigen-specific CD8+ T cells. Mice infected with Listeria monocytogenes (L. monocytogenes) expressing chicken ovalbumin as a model antigen mount ovalbumin-specific CD8+ T cell responses. Effector CD8+ T...

Tumor cell killing utilizing hematoporphyrin derivative (HPD) phototherapy was studied in a murine tumor model to assess the relative importance of intratumor temperature in causing the tumoricidal effect. Temperature measurements and tumor killing without HPD strongly suggest that HPD phototherapy as reported previously by other workers has at least partially a hyperthermic effect. Also, it is...