نتایج جستجو برای: key words pcr elisa
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Leishmaniasis is a disease caused by a malaria-like parasite called Leishmania in human and some species of animals. Detection of leishmaniasis has always been crucial for control and treatment of the disease. Different strategies have been approached for detection of leishmania. In this review methods used for detection of leishmania infection have been discussed and compared. Key word: Leishm...
The study recorded for the first time in Egypt presence of tomato spotted wilt virus (TSWV) on cucurbits, which was confirmed using several serological tests, like double antibody sandwich ELISA (DAS-ELISA); Dot-blot assay and Immuno capture polymerase chain reaction (IC-PCR). By Dot blot immunoassay test against TSWV antisera, ability thrips species Thrips tabaci (Lind), palmi (Karny), Frankl...
Vegetarianism is an old classic eating behavior that leads to lessening of several health risk factors. Vegetarian diet is reported to improve control of blood glucose concentration, to lower insulin requirement and to aid in weight control in diabetic patients. However, there is no evidence for the prevalence of diabetes mellitus to be significantly lower in vegetarians than in non-vegetarians...
هدف: در میان باکتریهای خانواده آنتروباکتریاسه، شیگلا دیسانتریه بدلیل تولید توکسین پروتئینی تحت عنوان شیگاتوکسین با داشتن فعالیت سیتوتوکسینی و نوروتوکسینی موجب بیماریهایی اسهال، گاستروانتریت و اختلال ا نعقاد داخل عروقی می شود که امروزه به عنوان مهمترین چالش برای سلامتی انسانها مطرح می باشند. هر چند روشهای کلاسیک ومتداول میکروبیولوژی برای تشخیص حساس واختصاصی می باشند اما محدودیت های خاص خود را ...
هدف: سالمونلا تیفی موریوم یکی از بیماری زاهای مهم غذایی است که باعث ایجاد گاستروانتریت می شود. در این تحقیق روش pcr-elisa برای شناسایی سالمونلا تیفی موریوم به کار گرفته شده است. مواد و روش ها: ژن rfb، که مسئول بیوسنتز آنتی ژن o از لیپوپلی ساکارید باکتری است، به عنوان توالی هدف انتخاب گردید. آغازگرهای تعیین شده منجر به تکثیر قطعه 882 جفت بازی برای گونه سالمونلا تیفی موریوم شد. نمونه های غذایی آل...
How much does language influence how we think? How far are the categories of our language contingent and culture-specific? Few questions are of greater significance to the social sciences. In this paper we attempt to demonstrate that linguistic semantics can address these questions with rigour and precision, by analysing some examples of cultural 'key words' in several languages. We want to arg...
This study explored touchscreen keyboards using high precision touchscreen strategies. Phase one evaluated three possible monitor positions: 30, 45, and 75 degrees from horizontal. Results indicate that the 75 degree angle, approximately the standard monitor position, resulted in more fatigue and lower preference ratings. Phase two collected touch bias and key size data for the 30 degree angle....
The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR ...
The use of an adequate concentration of Triton X-100 enhanced immunomagnetic separation of Coxiella burnetii from milk. PCR-enzyme-linked immunosorbent assay (PCR-ELISA) could detect coxiellas more sensitively than could conventional PCR. PCR-ELISA is therefore thought to be suitable for the simultaneous assay of a large number of samples. However, the number of cows from which raw milk tested ...
Infectious bovine rhinotracheitis (IBR) is a group of the respiratory disease multifaceted pathogens and key cattle, leading to significant economic losses dairy industry globally. The causative agent IBR Bovine herpesvirus type-1 (BoHV-1), which member genus Varicellovirus in Alphaherpesvirinae subfamily, belongs family Herpesviridae, order Herpesvirales. BoHV-1 can be categorized into three s...
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