BACKGROUND The mechanism behind the lowered postprandial insulin demand observed after rye bread intake compared to wheat bread is unknown. The aim of this study was to use the metabolomics approach to identify potential metabolites related to amino acid metabolism involved in this mechanism. METHODS A sourdough fermented endosperm rye bread (RB) and a standard white wheat bread (WB) as a ref...

A specially designed high-speed blendor and glass beads have been used to disintegrate yeast cells. The method enables large quantities of cells to be fragmented quickly at low temperature, and cell-free mitochondrial particles to be prepared in high yield. The particles are isolated in a sucrose-Tris-EDTA medium and extensively refractionated in the same medium. The success of the fractionatio...

We developed an enrichment medium for use with the loop-mediated isothermal amplification (LAMP) assay (enrichment media + LAMP assay) to quickly increase a very small number of Vibrio parahaemolyticus cells to the detection limit of the assay. Thirty-nine different enrichment media were prepared based on evaluating 12 potential ingredients. From our assessment of the 39 media, enrichment m...

Biochemical study on the metabolism of murine leprosy bacilli was first com menced by Hanks (1951). According to his report, the hydrogen transfer capacity of the bacilli was so feeble that only 0.8ƒÊmole of TTC (Triphenyl Tetrazolium Chloride) was reduced per mg nitrogen of the bacilli after 24 hours' incubation at 37•Ž. Soon after, Gray (1952) has found by manometric method that only 5 cmm of...

Abstract Herein, a novel sandwich-like α -ketoglutaric acid Schiff base-aminated chitosan composite ( -kGl-AmCsSB) was fabricated by reacting and aminated chitosan. The as-fabricated -kGl-AmCsSB inspected diversified characterization tools to determine its morphology, surface charge, chemical composition as well define the linkage pathway between -kGl AmCs. SEM images demonstrated spongy networ...

The modular pathway for the metabolism of 2,4-dichlorophenoxyacetic acid (2,4-D) encoded on plasmid pJP4 of Alcaligenes eutrophus JMP134 appears to be an example in which two genes, tfdA and tfdB, have been recruited during the evolution of a catabolic pathway. The products of these genes act to convert 2,4-D to a chloro-substituted catechol that can be further metabolized by enzymes of a modif...

Two isozymes of aspartate aminotransferase have been demonstrated biochemically. One isozyme is found in the mitochondrial fraction of the cytoplasm, the other ("soluble") in the supernatant. Both isozymes can be demonstrated by the cytochemical technique of Lee and Torack, as reported in the preceding report. Aldehyde fixation rapidly inactivates both isozymes, especially the soluble one. Inac...

It has been demonstrated (1) that the oxidation-reduction of methylglyoxal to lactic acid occurs in two steps. The first step is a condensation of methylglyoxal and reduced glutathione to a thiol ester which is hydrolytically cleaved to lactic acid and reduced glutathione. It was pointed out that such a mechanism might be operative in other aldehyde-oxidizing systems such as glyceraldehyde phos...

Ajl and Werkman (1948) have previously reported on the heterotrophic replacement of carbon dioxide and reviewed the literature on the bacterial requirement of the gas for optimal growth. A number of compounds have been found that in the case of both Escherichia coli and Aerobacter aerogenes substitute for carbon dioxide to give not only normal but, in many instances, enhanced growth. These comp...