Pulse-chase studies of [35S]cysteine-labeled fibrillin were performed on fibroblast strains from 55 patients with Marfan syndrome (MFS), including 13 with identified mutations in the fibrillin-1 gene and 10 controls. Quantitation of the soluble intracellular and insoluble extracellular fibrillin allowed discrimination of five groups. Groups I (n = 8) and II (n = 19) synthesize reduced amounts o...

Treatment for significant hypoglycemia includes administration of dextrose containing agents, including 50% dextrose (D50%W) intravenously. Significant extravasation of D50%W can lead to complications, including skin and soft tissue injury, loss of limb, or death. The aim of this case report, using an interdisciplinary team approach, explores extravasation protocols as well as literature review...

background and aims. the majority of complete denture wearers are old. clinical experience suggests that complete denture wearers have various disorders in their gustatory and olfactory senses due to disturbance of airways between the oral and nasal cavities caused by upper complete denture. the purpose of this study was to evaluate the effect of upper complete denture on gustatory and olfactor...

Background and Aims: This research aimed to study a possible link between endometriosis and polymorphism of the progesterone receptor gene. Materials and Methods: The control group consisted of 86 women without endometriosis and the case group comprised 86 patients with a diagnosis of endometriosis by laparoscopy. Genotypes for Alu insertion polymorphisms (A1/A1, A1/A2 and A2/A2) were descri...

The recJ gene encodes a single-strand DNA-specific exonuclease involved in homologous recombination. We have isolated a pseudorevertant strain in which recJ mutant phenotypes were alleviated. Suppression of recJ was due to at least three mutations, two of which we have identified as alterations in DNA helicase genes. A recessive amber mutation, "uvrD517am," at codon 503 of the gene encoding hel...

We created plasmids for use in insertion-duplication mutagenesis (IDM) of Neisseria gonorrhoeae. This mutagenesis method has the advantage that it requires only a single cloning step prior to transformation into gonococci. Chromosomal DNA cloned into the plasmid directs insertion into the chromosome at the site of homology by a single-crossover (Campbell-type) recombination event. Two of the ve...

OBJECTIVE To compare the viability of transferring the lower and transverse trapezius to the greater tuberosity using three different techniques. METHODS Twelve shoulders from six cadavers were used. The primary outcome was to assess the suture viability of the trapezius muscle transfer to the greater tuberosity in the insertion topography of the infraspinatus, with the arm adducted during in...

Background: Vulvovaginal Candidiasis is one of the most common vaginal infections and identification of candida species can be helpful in medical treatment. Intra uterine devices (IUDs) are effective, long-term methods of contraception, which in some cases may lead to Vulvovaginal Candidiasis. This study was developed to assess the effect of IUD insertion on Candida fungal flora in cervicovagin...

OBJECTIVE The vestibular implant seems feasible as a clinically useful device in the near future. However, hearing preservation during intralabyrinthine implantation remains a challenge. It should be preserved to be able to treat patients with bilateral vestibulopathy and (partially) intact hearing. This case study investigated the feasibility of hearing preservation during the acute phase afte...