As part of this laboratory’s honeybee genetics and breeding program, DNA markers are needed for uses such as the identification of populations and species and marker-assisted breeding. In particular, DNA markers are needed to discriminate Varroa-mite-resistant Russian honey bees, imported from fareastern Russia, and their offspring (1), from the other honey bees found in the USA. I tried RAPDs,...

ABSTRACT In the smut fungi, few features are available for use as taxonomic criteria (spore size, shape, morphology, germination type, and host range). DNA-based molecular techniques are useful in expanding the traits considered in determining relationships among these fungi. We examined the phylogenetic relationships among seven species of Ustilago (U. avenae, U. bullata, U. hordei, U. kolleri...

Erythropalum scandens Blume, an emerging medicinal plant with great potential for drug development, also possesses high edible value. In this study, we investigated the genetic diversity of germplasm E. obtained from different geographical locations using inter simple sequence repeat (ISSR) markers. For purpose, 18 ISSR primer pairs a distinct background and adequate polymorphism were selected....

Brycon is one of the main genera of Neotropical freshwater fish. In Brazil, Brycon species have been found in many hydrographic basins, such as the Amazon, Paraná, Paraguay, and Araguaia-Tocantins basins. We examined the phylogenetic relationships among the species Brycon orbignyanus, B. hilarii, B. cf. pesu, B. cephalus, B. falcatus, and B. gouldingi, using mitochondrial and nuclear molecular ...

Catharanthus roseus or vinca produces over 130 TIAs with pharmaceutical value. Two of them, namely vinblastine (VB) and vincristine (VC), are species-specific and extensively used in anticancer chemotherapeutics. To detect C. roseus genotypes abundant in desired TIAs, contrasting genotypes should be analyzed via marker-assisted selection (MAS) to avoid analytical quantitation. A trustable morph...

Genetic analysis of 30 accessions of marvel grass (Dichanthium annulatum Forsk.), a tropical range grass collected from grasslands and open fields of drier regions, was carried out with the objectives of identifying unique materials that could be used in developing the core germplasm for such regions as well as to explore gene (s) for drought tolerance. Five inter-simple sequence repeat (ISSR) ...

Rye (Secale cereale L.) is important food crop in Russia. The study of the genetic diversity crops carried out using molecular markers. To identify effective primers, ISSR (Inter Simple Sequence Repeats), a method for determining DNA polymorphism, was used.The selection primers according to largest number polymorphic fragments, their clear amplification and reproducibility repeated PCR. Of 22 a...

Background: Arecanut (Areca catechu L.) is an important plantation crop of India belongs to the family Arecaceae. As it a highly cross-pollinated there significant difference among genotypes. Morphological traits used for determining diversity and relationships genotypes or variety are prone environmental influences. Thus, usefulness molecular markers has been investigated as means characterizi...

Cucumber is an important vegetable crop grown worldwide. Genetic diversity and similarity among 11 cucumber genotypes viz. PCUCP-2, PCUCP-3, Nun-3139, Nun-3121, Nun-3141, Infinity, Isatis and Kian and PCUC-8, PCUC15 and PCUC-28 were grown under greenhouse conditions in G. B. Pant University of Agriculture and Technology, Pantnagar. Fresh and young leaves were collected from each genotype. The l...