نتایج جستجو برای: issr pcr

تعداد نتایج: 176786  

ژورنال: :تاکسونومی و بیوسیستماتیک 0
بهرام شریف نبی مسعود بهار مجید طالبی بدف احد یامچی

با توجه به تنوع بالای مورفولوژیکی در ارقام انار ایران، تنوع ژنتیکی 24 رقم انار با استفاده از نشانگرهای rapd و issr مورد بررسی قرار گرفت. آغازگرهای rapd در مجموع 131 قطعه dna تکثیر نمودند که 29 قطعه آنها (14/22 درصد) چندشکل بودند. آغازگرهای issr نیز از مجموع 173 قطعه تکثیر شده، 29 (37 درصد) چند شکلی حاصل نمودند. میانگین محتوای اطلاعات چندشکل (pic) برای آغازگرهای rapd و issr به ترتیب 128/0 و 163/...

Journal: :Journal of Insect Science 2004
Jesse H. de León Walker A. Jones David J. W. Morgan

Two molecular methods were utilized to distinguish geographic populations of Gonatocerus morrilli (Howard) from Texas and California and to test the possibility that this species could exist as a species-complex. Inter-Simple Sequence Repeat-Polymerase Chain Reactions (ISSR-PCR) were performed with a 5'-anchored ISSR primer. Twenty-five markers were generated with four populations (40 individua...

ژورنال: :دو فصلنامه تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران 2013
وجیهه قندهاری اسدالله احمدی¬خواه وحیده پیام نور

ارزیابی دقیق مقدار و پراکنش تنوع ژنتیکی در گونه های نادر و در معرض خطر به منظور تدوین راهبرد حفاظت و استفاده از منابع ژنتیکی از اهمیت ویژه­ای برخوردار است. به منظور مطالعه تنوع ژنتیکی بین و درون جمعیتی از سه جمعیت طبیعی شمشاد (buxus hyrcana pojark.) در شمال کشور (بندرگز، نوشهر و گرگان) و با استفاده از نشانگرهای issr استفاده شد. از هر جمعیت 20 نمونه تصادفی انتخاب گردید، dna آنها با استفاده از 10...

Journal: :Genetics and molecular research : GMR 2014
G F Cui L F Wu X N Wang W J Jia Q Duan L L Ma Y L Jiang J H Wang

Inter-simple sequence repeat (ISSR) markers were used to discriminate 62 lily cultivars of 5 hybrid series. Eight ISSR primers generated 104 bands in total, which all showed 100% polymorphism, and an average of 13 bands were amplified by each primer. Two software packages, POPGENE 1.32 and NTSYSpc 2.1, were used to analyze the data matrix. Our results showed that the observed number of alleles ...

2015
Carla A. Vitorino Renata C. C. Oliveira Vladimir P. Margarido Paulo C. Venere

The genetic diversity of the specimens of four natural populations of Arapaima from Araguaia-Tocantins basin was assessed within and among these stocks, using five primers for ISSR. COI (cytochrome c oxidase subunit I) partial sequences confirmed that the specimens belongs to Arapaima gigas. The ISSR provided 168 loci, of which 165 were polymorphic. However, the number of loci for each populati...

2016
Magdalena Szenejko Przemysław Śmietana Edyta Stępień

BACKGROUND Poa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link bet...

Journal: :Tree physiology 2005
T Balasaravanan P Chezhian R Kamalakannan M Ghosh R Yasodha M Varghese K Gurumurthi

Eucalyptus is the most economically important hardwood plantation tree cultivated in tropical and subtropical countries. Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic relationships within and between individuals of six Eucalyptus species. A total of 583 loci (265 to 1535 bp) were amplified from 149 individuals belonging to the six Eucalyptus species using seven ISSR ...

Journal: :Genetics and molecular research : GMR 2013
O N de Jesus J P X de Freitas J L L Dantas E J de Oliveira

We examined the genetic diversity of papaya (Carica papaya) based on morpho-agronomic and molecular data. Twenty-seven genotypes grown in Brazil were analyzed with 11 AFLP primer combinations, 23 ISSR markers, 22 qualitative, and 30 quantitative descriptors. For the joint analyses, we used the Gower algorithm (Joint Gower) and the average value of the individual dissimilarity matrix for each ty...

Based on the purpose of conservation planning for native species, sixteen populations of cattle, goat andsheep were analyzed by amplification of genomic DNA using inter-simple sequence repeat (ISSR) markers toestimate of genetic structure. DNA samples of 275 animals were collected to Paccess their genetic content.The polymorphism information content (PIC) values and genetic di...

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