Samples from the kidney of 100 diseased fish with signs of haemorrhagic septicemia and 50 samples from outlet water of fish farms were taken aseptically and cultured. In the laboratory, 75 colonies of gram-negative bacteria were biochemically diagnosed as motile aeromonads in our Bacteriology Laboratory Unit using API 20E rapid identification system. The genotype identification using specific p...

Introduction and Aim: Polymerase chain reaction (PCR) of 16S rRNA virulence genes associated with Type Three Secretion System (TTSS) has been used as a rapid method for the identification pathogen Enterobacter cloacae in leukemia patients. Rapid diagnosis this becomes necessary starting proper treatment these In current study, we aimed to isolate from patients study TSSS isolates using molecula...

The traditional cultural method (PCR and Real-Time PCR) for Salmonella spp. detection identification is laborious time-consuming. A qualitative LAMP detecting was validated in compliance with ISO 16140:2016. results show a relative accuracy, sensitivity, specificity of 100% comparison the reference 6579-1:2017; LOD50 set as 0.4 CFU/g. Additionally, field study carried out comparing kit, commerc...

How do observers detect the presence of objects or features in visual images? Stochastic stimuli (for example, white noise) have become popular choices for providing a linear characterization of early sensory mechanisms. A recent paper by Neri and Heeger takes this type of methodology a step further, and succeeds in isolating and characterizing non-linear mechanisms responsible for the detectio...

There is an optimal way of increasing certain cardinal invariants of the

This study was conducted to determine how the isolation method of the porcine preantral follicles influenced the following follicular growth in vitro. Mechanical and enzymatical isolations were used for retrieving the follicles from prepubertal porcine ovaries, and in vitro-growth of the follicles and the expression of folliculogenesis-related genes were subsequently monitored. The enzymatic re...

four halotolerant fungal isolates originating from the saltwater lake urmia in iran were selected during a screening program for salt resistance and α-amylase activity. the isolates were identified based on sequencing the its region and a part of the β-tubulin gene, as penicillium chrysogenum (isolate u1; cbs 132820), fusarium incarnatum (isolate u2; cbs 132821), and penicillium polonicum (isol...

INTRODUCTION Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest....

We investigated the characteristics of mechanisms mediating motion discrimination of S-cone isolating stimuli and found a double dissociation between the effects of luminance noise, which masks linear but not non-linear motion, and chromatic noise, which masks non-linear but not linear motion. We conclude that S-cones contribute to motion via two different pathways: a non-linear motion mechanis...

The majority (>99%) of microorganisms from the environment resist cultivation in the laboratory. Ribosomal RNA analysis suggests that uncultivated organisms are found in nearly every prokaryotic group, and several divisions have no known cultivable representatives. We designed a diffusion chamber that allowed the growth of previously uncultivated microorganisms in a simulated natural environmen...