Column chromatography of the Egyptian date palm pollen, resulted in the isolation of estradiol, estrone among ten compounds from the hexane fraction. Also, besides rutin, additional four flavonoids from the ethyl acetate extract were isolated. HPLC analysis of the hexane fraction (using RFBDS C18 – isocratic acetonitrile /water 58:42) revealed the presence of estrone, estradiol and estriol comp...

The separation of myo-inositol phosphates by ion pair, reverse-phase high performance liquid chromatography has been investigated. The retention of the inositol phosphates is dependent on both the polarity of the hetaeron utilized and on the pH of the solvent. A method is presented which permits the isocratic separation of multiple forms of inositol phosphates including isomers of myo-inositol ...

This HPLC method for measuring antipyrine, lorazepam, and indocyanine green in 0.5 mL of plasma can be used in studies of liver function in which these "model" compounds are used. After a fast, simple, one-step extraction procedure with acetonitrile, an isocratic HPLC system is used, with a single detection wavelength (214 nm) and a single internal standard (1-acetamidopyrene). The mobile phase...

A sensitive accurate, fast and precise isocratic reverse phase high performance liquid chromatographic method has been developed for the determination of prulifloxacin in bulk and in tablet dosage form. The developed method was found to be simple and have short run time which makes the method rapid. Statistical analysis proves that method is repeatable and selective for the analysis of pruliflo...

A sensitive, precise, specific, linear and stability indicating isocratic HPLC method was developed for the analysis of related substances in zolmitriptan. The potential known related substances are (S)-4-(4-aminobenzyl)-1,3-oxazolidin-2-one (impurity I) and (S)-4-(4-hydrazinobenzyl)-1,3-oxazolidin-2-one (impurity II). The method can be used for the detection and quantification of known and unk...

An isocratic RP-HPLC method was developed for the estimation of atropine sulphate in bulk. The separation was achieved with a C18 column using mobile phase comprising of methanol:5 mmol potassium dihydrogen phosphate buffer (50: 50, v/v), the pH of which was kept unadjusted. The flow rate was kept at 1 mL min-1 and analysts was screened with UV detector at 264 nm. The retention time for atropin...

A new automated, qualitative screening HPLC method for the identification of basic compounds in urine has been established. A 1-ml volume of urine was automatedly extracted online and separated on two coupled strong cation-exchange columns under isocratic conditions. The use of the photodiode-array detector (DAD, λ=190-370 nm) gave access to a library of more than 2600 toxicologically relevant ...

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 x 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water:isopropylalcohol (22:18:68:2; adjusted to pH 8.0 using triethylamine; v/v) was used. The flow ra...