The kidney plays an important role in ion regulation in both freshwater and seawater fish. However, ion transport mechanisms in the teleost kidney are poorly understood, especially at the molecular level. We have cloned a kidney-specific SLC26 sulfate/anion exchanger from rainbow trout (Oncorhynchus mykiss) that is homologous to the mammalian SLC26A1 (Sat-1). Excretion of excess plasma sulfate ...

Cultured human monocytes released cytostatic activity upon in vitro activation with lymphokines and lipopolysaccharide. This activity was mainly due to the presence of two different cytostatic factors, termed CstF I and II, which were separated by ion-exchange chromatography. At neutral pH, CstF I bound to the weak anion exchanger DEAE-Sephacel but not to the weak cation exchanger CM-Sepharose,...

Two mechanisms of H+ ion secretion in the proximal tubule that mediate bicarbonate reabsorption have been identified: the brush border Na/H exchanger and electrogenic H+ ion secretion. Angiotensin II (AII) has been shown to be a regulator of the luminal Na+/H+ exchanger and the basolateral Na+/HCO3- cotransporter. In the present study, we examined the effects of AII on H+-ATPase activity in iso...

Threonyl ribonucleic acid synthetase was purified 400-fold from rat liver. During stepwise chromatography on diethylaminoethyl cellulose, two fractions with this enzymatic activity were observed. The separated fractions give distinct elution patterns on rechromatography on the same ion exchanger. Threonyladenylate-enzyme complex prepared with 14Cthreonine and 3H-adenosine triphosphate shows a s...

N-propylsalicylidene based on silica as ion exchanger (IE) was used for the separation by complexation of Mn2+, Co2+, Ni2+, Cu2+, Hg2+, Cr3+, Fe3+, and UO22+ from their parent solutions. IE and its metal complexes are characterized by elemental analysis, electronic and infrared spectra, in addition to thermal analysis in atmospheric pressure. The mode of chelation and the proposed geometric str...

Vectorial ion transport initiated by Na+/H+ exchanger isoform 3 (NHE3) mediates the reabsorption of NaCl and NaHCO(3) in renal proximal tubule cells. NHE3 activity is modulated by numerous physiological stimuli. Biochemical and cellular experiments identified Na+/H+ exchanger regulatory factor (NHERF) as a protein cofactor essential for cAMP-mediated inhibition of NHE3 activity. Identification ...

The invention deals with a novel method for immobilization enzymes on solid surfaces such as metallic and graphite ones, which overcomes the disadvantages of standard current methods. Technology The immobilization technique is accomplished by doping the surface with a blend of the enzymes to be fixed, glutaraldehyde and nafion-ion exchanger, as protective additive. Therefore, the above techniqu...

A fall in extracellular pH increased membrane conductance of the giant cell in the abdominal ganglion of Aplysia californica. Chloride conductance was trebled whereas potassium conductance was increased by 50%. Half the giant cells were hyperpolarized (2-8 mv) and half were depolarized (3-10 mv) by lowering the pH. The hyperpolarizing response always became a depolarizing response in half-chlor...

Investigation of malonyl-CoA:acyl carrier protein transacylase from soybeans has shown that this fatty acid biosynthetic enzyme occurs in at least two isozymic forms. Both forms exist as soluble, low-molecular-mass polypeptides (approx 43 kDa) which catalyze one of the first committed steps in the synthesis of C16 and CIS fatty acids. We have partially purified the two forms of this enzyme from...

Abstract The interest for ZrP this material is based on its physicochemical properties which makes a perspective candidate applications in nuclear medicine. In study was prepared and completely characterized using various analytical methods. Finally, the of radiometals sorption mechanism surface characterization were done. conclusion, appears as promising next studies with purposes like drug de...