The use of tetramethylammonium hydroxide (T.M.A.H.) in place of potassium hydroxide in the Zimmermann reaction has been investigated. Although various pure steroids have different colour equivalents, a comparison of the results of 17-ketosteroid and 17-hydroxycorticosteroid estimations on a series of urines showed that the differences for the two reagents were only small and may be ignored. Tet...

The urinary excretion of androsterone, aetiocholanolone, total 17-oxosteroids, and 17-hydroxycorticosteroids (17-OHCS) was measured in 40 patients with lung cancer three days before resection and again 10-15 days after resection of their lung tumours. There was a significant postoperative increase in the excretion of 17-OHCS but a significant decrease in the excretion of androsterone and aetioc...

We examined the effect on some clinical chemical assays of adding chlordiazepoxide or its two metabolites, the lactam and the N-desmethylchlordiazepoxide, to serum or urine. We also examined the effect on urinary steroid values of administering the drug for as long as 20 days. The drug, in 10-fold the expected concentrations in blood, or its metabohites, in 200-fold the expected Concentration i...

The determination of urinary corticosteroids by their conversion to 17-ketosteroids (17-KS) on treatment of urine with sodium bismuthate has been recently reported (Norymberski, 1952; Norymberski, Stubbs, and West, 1953). The group of corticosteroids thus determined has been termed 17-ketogenic steroids (17-KGS). The interest in this method shown by colleagues from other laboratories prompts us...

Previous studies have shown that IL-17 is a strong proinflammatory cytokine and that IL-17-producing autoreactive T cells play a major role in the pathogenesis of autoimmune diseases. In a previous study, we showed that injection of experimental autoimmune uveitis-susceptible mice with anti-IL-17 Abs blocked subsequent disease development. To determine whether administration of IL-17 to experim...