The characterization of protein interactions is essential for understanding biological systems. While genome-scale methods are available for identifying interacting proteins, they do not pinpoint the interacting motifs (e.g., a domain, sequence segments, a binding site, or a set of residues). Here, we develop and apply a method for delineating the interacting motifs of hub proteins (i.e., highl...

Transcriptional coregulators contribute to several processes involving nuclear receptor transcriptional regulation. The transcriptional coregulator androgen receptor-interacting protein 4 (ARIP4) interacts with nuclear receptors and regulates their transcriptional activity. In this study, we identified p62 as a major interacting protein partner for ARIP4 in the nucleus. Nuclear magnetic resonan...

amyloid pathology is associated with fibril aggregation of different proteins which results in the progressive damage of affected organs. it is strongly believed that specific small molecules interfere with fibrillation by interacting with the amyloidogenic proteins. we had previously reported the strong and long-term inhibition of fibrillation of hen egg white lysozyme (hewl) by cuminum cyminu...

As sessile organisms, plants possess a complex system to cope with environmental changes. Ca2+ functions as vital second messenger in the stress signaling of plants, and CBL-interacting protein kinases (CIPKs) serve essential elements plant pathway. In this study, calcineurin B-like protein-interacting kinase 26 (BdCIPK26) from Brachypodium distachyon was characterized. Overex...

BACKGROUND Hepatitis B Virus (HBV) DNA polymerase transactivated protein 1 (HBVDNAPTP1) is a novel protein transactivated by HBV DNA polymerase, screened by suppression subtractive hybridization technique (GenBank accession no: AY450389). The biological function of HBVDNAPTP1 was investigated in this study. METHODS We constructed a vector pcDNA3.1 (-)/myc-His A-HBVDNAPTP1 and used it to trans...