Insecticide resistance can arise from a variety of mechanisms, including changes to the target site, but is often associated with substantial fitness costs to insects. Here we describe two resistance-associated target-site mutations that have synergistic and compensatory effects that combine to produce high and persistent levels of resistance to fipronil, an insecticide targeting on γ-aminobyty...

Metal exposure is one of the commonest anthropogenic pollutants mosquito larvae are exposed to, both in agricultural and urban settings. As members of the Anopheles gambiae complex, which contains several major malaria vector species including An. arabiensis, are increasingly adapting to polluted environments, this study examined the effects of larval metal exposure on various life history trai...

Malaria transmission has been substantially reduced across Africa through the distribution of long-lasting insecticidal nets (LLINs). However, the emergence of insecticide resistance within mosquito vectors risks jeopardizing the future efficacy of this control strategy. The severity of this threat is uncertain because the consequences of resistance for mosquito fitness are poorly understood: w...

The origin and insecticide resistance status of Diamondback moth (Plutella xylostella) populations infesting canola, vegetables, forage brassicas and weeds has fundamental implications for management of the pest in Australia. Diamondback moth is considered a migratory species in the Northern Hemisphere, but although there is evidence that Diamondback moth moves en-masse throughout Australia in ...

In order to improve the chemical control of diamondback moth, Plutella xylostella (L), in Taiwan, the Taiwan Agricultural Research Institute organized in 1980 a program aimed at analyzing the insecticide resistance and mode of action. After almost five years of study, we have gathered information on the sampling method, resistance, cross resistance, the mode of insecticide action, and the effic...

The development of insecticide resistance in Asian citrus psyllid, Diaphorina citri Kuwayama, populations is a serious threat to the citrus industry. As a contribution to a resistance management strategy, we developed a glass vial technique to monitor field populations of Asian citrus psyllid for insecticide resistance. Diagnostic concentrations needed to separate susceptible genotypes from res...

the house fly, musca domestica l., is known as one of the most important hygiene problems worldwide. it has shown a high potential to develop resistance to chemical insecticides. this study was undertaken to determine the susceptibility or resistance of the house fly, which were collected from 3 livestock farms near the city of ahvaz, to prevalent pyrethroid insecticides (delthamethrin, lambda-...

The emergence of insecticide resistant Aedes aegypti mosquitoes has hampered dengue control efforts. WHO susceptibility tests, using several pyrethroid compounds, were conducted on Ae. aegypti larvae that were collected and raised to adulthood from Semarang, Surakarta, Kudus and Jepara in Java. The AaNaV gene fragment encompassing kdr polymorphic sites from both susceptible and resistant mosqui...

Malaria control programs are being jeopardized by the spread of insecticide resistance in mosquito vector populations. The situation in Burkina Faso is emblematic with Anopheles gambiae populations showing high levels of resistance to most available compounds. Although the frequency of insecticide target-site mutations including knockdown resistance (kdr) and insensitive acetylcholinesterase (A...

G-protein-coupled receptors regulate signal transduction pathways and play diverse and pivotal roles in the physiology of insects, however, the precise function of GPCRs in insecticide resistance remains unclear. Using quantitative RT-PCR and functional genomic methods, we, for the first time, explored the function of GPCRs and GPCR-related genes in insecticide resistance of mosquitoes, Culex q...