the aphid species, cinara pini (linnaeus, 1758) reported in our previous work as a new aphid on pinus trees for iran, was described using the classic method and through analysis of coi gene sequence. in the next step, we addressed the efficiency of the entomopathogenic fungus, lecanicillium longisporum (zimm.) zare and gams strain lrc 190, on the aphid. the fungus was administered to the second...

zoonotic cutaneous leishmaniasis (zcl) is an increasing and important public health problem in iran. the use of repellents is recommended as one of the important means of personal protection against vectors of zcl. this paper reports the repellency effect of the plant myrtle, myrtus communis (myrtaceae), essential oil for protection against 3-7-day-old unfed females of the sandfly, phlebotomus ...

background and aims: virus-like particles (vlps) have been suggested to be a promising recombinant vaccine approach. several studies have reported that the influenza vlps produced in insect cells is an effective vaccine candidate. due to crucial role of matrix m1 protein in assembly and budding of influenza particles, in all vlps structures, m1 protein have been considered as a main component. ...

The baculovirus expression vector system has been commonly employed as a rapid and inexpensive approach for recombinant protein production in insect cells (1, 2). Despite the wide applications, baculovirus infection results in insect cell death and lysis in a few days post-infection, which can lead to suboptimal protein processing late in the infection phase. In addition, some proteins that are...

Baculoviruses are insect viruses extensively exploited as eukaryotic protein expression vectors. Molecular biology studies have provided exciting discoveries on virus-host interactions, but the application of omic high-throughput techniques on the baculovirus-insect cell system has been hampered by the lack of host genome sequencing. While a broader, systems-level analysis of biological respons...

The insect immune response has a number of structural and functional similarities to the innate immune response of mammals. The objective of the work presented here was to establish the mechanism by which insect hemocytes produce superoxide and to ascertain whether the proteins involved in superoxide production are similar to those involved in the NADPH oxidase-induced superoxide production in ...

Insect gustatory receptors are predicted to have a seven-transmembrane structure and are distantly related to insect olfactory receptors, which have an inverted topology compared with G-protein coupled receptors, including mammalian olfactory receptors. In contrast, the topology of insect gustatory receptors remains unknown. Except for a few examples from Drosophila, the specificity of individu...

Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a more efficient method that can replace the existing method. This method is economical and does not require any special a...

Baculovirus-mediated expression has become a powerful tool for the high yield production of functionally active recombinant proteins. In order to further enhance the utility of this expression system, we constructed versatile transfer vectors that facilitate the secretion of recombinant proteins from host insect cells by inserting functional secretory leader sequences down-stream of the polyhed...

Insect cell lines have been widely used in recombinant baculovirus expression systems and transient gene expression studies. Critical to these applications have been the transfection of foreign DNA. This has been frequently done using labor intensive and cytotoxic liposome-based transfection reagents. In the current study we have optimized a new kind of polyethylenimine-based DNA transfection r...