Inhibition of purified AMP-deaminase from chicken breast muscle by phosphate esters and inorganic phosphate has been investigated. When tested at a concentration of 3 mM, in the presence of 0.15 M potassium chloride, the order of effectiveness as inhibitors of deaminase activity was: 2,3diphosphoglyceric acid, 3-phosphoglyceric acid, 2-phosphoglyceric acid, 2-phosphoenolpyruvate, fructose 1,6di...

Vascular calcification is a common finding in atherosclerosis and a serious problem in diabetic and uremic patients. Because of the correlation of hyperphosphatemia and vascular calcification, the ability of extracellular inorganic phosphate levels to regulate human aortic smooth muscle cell (HSMC) culture mineralization in vitro was examined. HSMCs cultured in media containing normal physiolog...

The large scale, respiration-linked accumulation of Ca+f ions by isolated mitochondria (l-lo), which leads to deposition of amorphous granules of calcium phosphate (5, 7, 8, lo-12), has been found to be accompanied by accumulation of significant amounts of a phosphate derivative (or derivatives) hydrolyzed to inorganic phosphate by boiling in 1 N HCl for 7 minutes (5). In this paper it is demon...

Lanthanide ions can mediate both phosphomonoester hydrolysis and beta-elimination of inorganic phosphate from polypeptide substrates under near-physiological conditions of pH, temperature, and salt.

Limitation of inorganic phosphate in the culture medium allows stalk elongation in wild-type Caulobacter crescentus. Mutants unable to form long stalks were isolated.

Hexametaphosphate (HMP) is an inorganic condensed phosphate, which has been used in a wide variety of industries for nearly century, originally patented to deflocculate clay and soften hard water...

1. Pea-seed phosphofructokinase was purified 27-fold by a combination of fractionation with ethanol and ammonium sulphate. Under the conditions of assay, the enzyme was strongly inhibited by phosphoenolpyruvate. This inhibition was reversed by increasing the concentration of fructose 6-phosphate or magnesium chloride, or by lowering the ATP concentration. 2. Citrate, ADP and AMP inhibited phosp...

Tetrahymena pyriformis, a freshwater protozoan, is common in aquatic systems. Arsenic detoxification through biotransformation by T. pyriformis is important but poorly understood. Arsenic metabolic pathways (including cellular accumulation, effluxion, biomethylation, and volatilization) of T. pyriformis were investigated at various phosphate concentrations. The total intracellular As concentrat...

Recently, we reported the production of Cadmium sulfide (CdS) fluorescent semiconductor nanoparticles (quantum dots, QDs) by acidophilic bacteria of the Acidithiobacillus genus. Here, we report that the addition of inorganic phosphate to Acidithiobacillus thiooxidans ATCC 19703 cultures favors the biosynthesis of CdS QDs at acidic conditions (pH 3.5). The effect of pH, phosphate and cadmium con...

It has recently been reported by Doudoroff, Barker, and Hassid (1) t’hat an exchange occurs between the phosphate group of glucose-l-phosphate and inorganic phosphate in the presence of sucrose phosphorylase and in the absence of acceptor. On the basis of this exchange, they postulate a mechanism involving the formation of an enzyme-glucose complex, thus endowing the enzyme wit,h the ability to...