A study, consisting of three trials, was undertaken in order to find standard reference sera for use in serological diagnostic procedures for infectious bovine rhinotracheitis (IBR) in the European Union (EU). A total of forty-nine laboratories participated in the trials, in which panels of positive, weak positive, and negative candidate sera were tested blind by neutralisation, indirect and bl...

The manufacturing process in Australia for equine antisera against various venoms/toxins is based primarily on ammonium sulphate precipitation of pepsin-digested IgG, whereby Fc and F(ab')(2)fragments are separated. The capacity of the process to remove non-enveloped and enveloped model viruses was assessed using a scaled-down process. Each virus was added to mid-process samples from equine pla...

Dr. Aradhana (“Ara”) Gupta has joined the Kansas State Veterinary Diagnostic Lab (KSVDL) as an instructor in clinical pathology. She is originally from Punjab state of India, which is famous for agriculture and Punjabi music. She graduated from Punjab Agricultural University, India in 2000 and then completed a master’s in epidemiology and preventive veterinary medicine in 2002. Her research foc...

This case report involves four dairies in the Willamette Valley, Oregon, which experienced reproductive problems associated with the presence of a large, previously unidentified, peak eluting at 5 min in a standard ergovaline high-performance liquid chromatography assay of perennial ryegrass silage fed to those animals. Mycotoxin analysis of the silage was negative, as was serological screening...

The UCD community has made this article openly available. Please share how this access benefits you. Your story matters! (@ucd_oa) Some rights reserved. For more information, please see the item record link above. Introduction The quality and safety of Irish agricultural product will become increasingly important, if Ireland is to remain competitive in a global trading environment. Animal healt...

The present study involved in analysis of 81 sera samples collected from Murrah (34), Surti (20) and non-descript buffaloes (27) with history of respiratory and reproductive disorders for IBR antibodies by indirect Enzyme linked immunosorbent assay (ELISA), Virus neutralization test (VNT) and Passive haemagglutination assay (PHA). The immunological assays viz., indirect ELISA, VNT and PHA revea...

Infectious bovine rhinotracheitis (IBR) virus was grown in Madin Darby bovine kidney (MDBK) cell line using a roller culture system for its large-scale production. Optimum multiplicity of infection (MOI) of 1:750 was found to give consistent virus yield. To determine the appropriate payload, three batches of antigen with virus titres ranging from 10(8.37) to 10(6.37) TCID50 per ml were used to ...

Three experiments were conducted using feedlot steers in a randomized block design to determine the effect of zinc methionine (ZnMet) and zinc oxide (Exp. 3) on feed intake (DMI), rectal temperature, and serum mineral concentrations of feedlot cattle challenged with infectious bovine rhinotracheitis virus (IBRV). All the steers used were seronegative to IBRV. Steers were adapted for 7 d to thei...