A tissue culture technique for micropropagation of chia (Salvia hispanica L.) was established. High percentage of seed germination (100%) was recorded on Murashige and Shoog medium enriched with 0.4 mg l gibberellic acid and 10 mg l ascorbic acid after one week of culture. The maximum number of shoots per explant (2.7) gave MS medium with 2 mg l 6-benzylaminopurine after five weeks of culture. ...

The present work demonstrates the participation of polyamines (PAs) to improve direct regeneration and Agrobacterium-mediated transformation in soybean half-seeds. The inclusion of PAs to culture medium along with optimal plant growth regulators (PGRs) enhanced shoot induction [98.3 %; 4.44 µM N6-benzyladenine (BA) and 103.27 µM spermidine] and elongation [90.0 %; 1.45 µM gibberellic acid (GA3)...

The Arabidopsis chy1 mutant is resistant to indole-3butyric acid, a naturally occurring form of the plant hormone auxin. Because the mutant also has defects in peroxisomal -oxidation, this resistance presumably results from a reduced conversion of indole-3-butyric acid to indole-3-acetic acid. We have cloned CHY1, which appears to encode a peroxisomal protein 43% identical to a mammalian valine...

A protocol has been developed for the direct induction of adventitious shoots from leaf explants of in vitro raised Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. The Murashige and Skoog (MS) media supplemented with N–benzyladenine (BA), kinetin (KIN), and thidiazuron (TDZ) were used in regeneration experiments. BA at 0.5 mg L concentration was chosen as the optimal treat...

Secondary metabolites derived from benzoic acid (BA) are of central importance in the interactions of plants with pests, pathogens, and symbionts and are potentially important in plant development. Peroxisomal b-oxidation has recently been shown to contribute to BA biosynthesis in plants, but not all of the enzymes involved have been defined. In this report, we demonstrate that the peroxisomal ...

Crataeva religiosa G. Forst. var. nurvala (Buch.-Ham.) Hook. F. & Thomson (syn. Crataeva nurvala Buch.-Ham.), a fast growing medicinal tree species, has been successfully micropropagated under in vitro conditions. Direct multiple shoots were regenerated from nodal explants on Murashige and Skoog’s (MS) medium, solidified by agar and supplemented with 1 mg/L 6-benzyl amino purine (BAP) and 0.5 m...

High demand for edible bamboo shoots of Bambusa tulda and Melocanna baccifera in many Asian ethnic groups has led to the need for developing intensive bamboo farming. To achieve this, in vitro regeneration of bamboo plantlets is needed due to the long and irregular bamboo flowering cycle and scarcity of bamboo seeds. An effective protocol for plantlets regeneration in B. tulda and M. baccifera ...

A suitable protocol for micropropagation of Casuarina hybrid, Casuarina equisetifolia L. 9 Casuarina glauca Sieber ex Spreng (C. e. 9 C. g.), was developed. When seeds without seed coats were cultured on 4 germination media, the optimal seed germination percentage (91%) was obtained on 0.8% agar solidified water medium. Shoot multiplication was achieved by culturing 2-cm long epicotyls, excised...