Carboxylesterases are essential enzymes in the hydrolysis and detoxification of numerous pharmaceuticals and pesticides. They are vital in mediating organophosphate toxicity and in activating many prodrugs such as the chemotherapeutic agent CPT-11. It is therefore important to study the catalytic mechanism responsible for carboxylesterase-induced hydrolysis, which can be accomplished through th...

BACKGROUND Halotolerant bacteria are excellent sources for selecting novel enzymes. Being intrinsically stable and active under high salinities, enzymes from these prokaryotes have evolved to function optimally under extreme conditions, making them robust biocatalysts with potential applications in harsh industrial processes. RESULTS A halotolerant strain LY19 showing lipolytic activity was i...

the german cockroach is an important household insect pest mechanically involved in transmission of a variety of diseases to humans. different classes of insecticides have extensively been used for its control leading to insecticide resistance development. hence, for an optimal control of this pest, the status and underlying mechanisms of insecticide resistance should be studied in this group o...

An increasing number of teachers are introducing animals into their class so that pupils foster cognitive, physiological, and social skills through their interaction with animals. Along with such an educational style termed animal-assisted education (AAE), Japanese formal education has also utilized animals for education. Japanese animal-rearing education is unique regarding the following two p...

Proteolytic activity, acid phosphatase activity and fructose concentration were measured in seminal plasma from 205 human semen samples. Certain routine sperm characteristics were also determined. High fibrinolytic activity was found and TAME-esterase and proteinase activity was also present in all specimens. The activity of all the proteolytic enzymes correlated positively with the acid phosph...

In this study, we determined the in vitro inhibitory effects of ceftriaxone sodium, imipenem and ornidazole on hydratase and esterase activities of human erythrocyte carbonic anhydrase-I and II isozymes (CA I and II). Human erythrocyte CAI and II isozymes were purified by Sepharose-4B L-tyrosine affinity chromatography column with a yield of 30% and 40%, a specific activity of 920 and 8,000 EU/...

this study focused on use of bacterial extracellular enzyme activities as biochemical parameters, along with the microbiological and physicochemical characteristics, in a comprehensive assessment of water quality of four lake ecosystems in the province of vojvodina (northern serbia): provala, ludas, zobnatica and palic. water samples were collected in june and october, 2008. for assessment of ...