Abstract The objective of this work was to develop a method create and validate CRISPR-Cas systems different gRNAs in soybean (Glycine max) embryos. Two model genes were used for simple mutation with one gRNA or partial gene deletion two guides. inserted into the CRISPR transformation vectors by type IIS restriction enzyme subcloning inserting promoter + gRNA2 final vector using classic cloning...

variants. Consequently, phyB may be an attractive candidate for allele-mining and targeted breeding approaches to reduce, or enhance, thermomorphogenic growth responses in crop species in efforts to breed climate warming-resilient cultivars. These and other matters will undoubtedly be addressed in future research endeavours following the milestone discovery of phyB acting [102_TD$DIFF]as a ther...