Carbonic anhydrases catalyze the interconversion of carbon dioxide to bicarbonate. Human carbonic anhydrase isozyme III with a C-terminal hexahistidine tag was overexpressed in Eschericha coli, purified and crystallized. Diffraction data (93.4% completeness) were collected to 2.2 A resolution on an in-house R-AXIS IV++ image-plate system with Osmic mirrors and a Rigaku HU-H3R CU rotating-anode ...

Fusion-tag affinity chromatography is a key technique in recombinant protein purification. Current methods for protein recovery from mammalian cells are hampered by the need for feed stream clarification. We have developed a method for direct capture using immobilized metal affinity chromatography (IMAC) of hexahistidine (His6) tagged proteins from unclarified mammalian cell feed streams. The p...

Since immobilized metal ion affinity chromatography (IMAC) was first reported, several modifications have been developed. Among them, Ni(2+) immobilized by chelation with nitrilotriacetic acid (NTA) bound to a solid support has become the most common method for the purification of proteins carrying either a C- or N-terminal histidine (His) tag. Despite its broad application in protein purificat...

The present study sets out to investigate whether the position of literary works in the English literary polysystem influences the Iranian translators’ translational behavior at the textual level. Given the prominent position of canon and bestseller novels in English literary polysystem, the study intends to find out whether the translators of canon and bestseller novels are faithful to theirso...

Malloy, Amanda, "Scout's daughters : race and creative development in contemporary adolescent literature" (2011). Honors Theses. Paper 117. At the heart of what Roberta S. Trites titles ―adolescent literature‖ – works written both for and about young adults—is a question of agency (Disturbing 7). In Disturbing the Universe: Power and Repression in Adolescent Literature, Trites asserts that adol...

one of the most outstanding authors and poets of algeria is kateb yasin who revealed his unfortunate country’s condition toward people. he has several trace of writing to french language in novel, poem and scenario fields. but spirit based on his work, mixing with islamic culture, is arabic spirit originated from aljazeera culture. the most important of his novels, which is a new experience in ...

This article discusses the position of novels Hari-hari Terakhir Seorang Seniman (1979) and Arus (1985) by Anwar Ridhwan as these are considered to be cultural products behaviors Malay community. Based on interpretation novels, from an ethnosemantics study Goodenough proves that (literature) collective knowledge system society in 1940s 1970s. In two has created a discourse phenomena discuss var...

An important goal is to identify the direct activation domain (AD)-interacting components of the transcriptional machinery within the context of native complexes. Toward this end, we first demonstrate that the multisubunit TFIID, SAGA, mediator, and Swi/Snf coactivator complexes from transcriptionally competent whole-cell yeast extracts were all capable of specifically interacting with the prot...

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Rhp14 of Schizosaccharomyces pombe is homologous to human XPA and Saccharomyces cerevisiae Rad14, which act in nucleotide excision repair of DNA damages induced by ultraviolet light and chemical agents. Cells with disrupted rhp14 were highly sensitive to ultraviolet light, and epistasis analysis with swi10 (nucleotide excision repair) and rad2 (Uve1-dependent ultraviolet light damage repair pat...