FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...

Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

rational explanation of the doctrine of imamat (spiritual leadership of the shi`ites) is one of the most important and efficient instruments that can prepare the ground for extension and propagation of this view in all over the world. so, in this article i want to study one of the first rational explanation of this view in islamic world which proposed by al- farabi. in the world of islam, farab...

baha’ al-dawlah razi was one of the most important physicians in safafid dynasty in iran. he wrote his large medical book, kholasat al-tajarob at 1501 ad. he worked as physician in the courts of two dynasties, teimurid and safavid. he had interesting innovations and findings in surgery, immunology and also in treatment. as examples, we can note to his description of pertussis and also his oral ...