A method for the titration of antigens based on separation by filtration through millipore filters of the complex of antigen with fluorescein isothiocyanate-labeled antibodies from free labeled anti­ bodies and nonspecific y-globulins is described. The fluorescence of the antigen-antibody complex is measured directly on the millipore filters using the Turner fluorometer equipped with a paper ch...

Maternal antibodies are transferred from hens to the chicks via the egg. To gain insight into maternal antibody transfer and endogenous production of antibodies in broiler chicks, total IgY, IgA, IgM, as well as anti-Newcastle disease virus (NDV) and anti-infectious bronchitis (IBV) antibody levels were examined in the dams' plasma, egg yolks, egg whites, and chicks' plasma on d 3, 7, 14, and 2...

Bering E. proposed the principle of passive immunization at end 19th century. Today, it is still used to treat tetanus, diphtheria, botulism, rabies and poisonous animal bites (snakes, spiders scorpions). As before, equine antibodies or their fragments are as an antidote. But unique properties from yolks chicken eggs (IgY) make possible use them for a wide range therapeutic prophylactic purpose...

In order to replace the use of mammals for the production of hyperimmune sera and to replace Freund"s complete adjuvant (FCA) with its undesirable side effects, the production and immunological features of IgY were investigated systematically. An important advantage of IgY is that large amounts can easily be extracted from the yolk of immunized laying hens. With special regard to animal welfare...

Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion, immunoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion and immunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of ma...

The objective of this study was to develop an economical, simple, and large-scale separation method for IgY from egg yolk. Egg yolk diluted with 9 volumes of cold water was centrifuged after adjusting the pH to 5.0. The supernatant was added with 0.01% charcoal or 0.01% carrageenan and centrifuged at 2,800 x g for 30 min. The supernatant was filtered through a Whatman no. 1 filter paper and the...

Avian eggs contain a variety of maternally-derived substances that can influence the development and performance of offspring. The levels of these egg compounds vary in relation to environmental and genetic factors, but little is known about whether there are correlative links between maternal substances in the egg underlying common and different pathways of maternal effects. In the present stu...