Dermatan sulfate is a naturally occurring antithrombotic glycosaminoglycan. The antithrombin activity of several dermatan sulfate preparations has been measured in whole human plasma and found to be -55% of that in purified systems. Kinetic studies under pseudo-first-order conditions indicated that the reduction in antithrombin activity of dermatan sulfate in plasma compared with that in buffer...

Microfluidic devices have been used for in-vitro studies in the past while recently they have been extended for in-vivo studies in whole organisms. In this report we use a microfluidic device for high resolution fluorescence imaging of axonal transport in model systems Caenorhabditis elegans and Drosophila larvae. The device immobilized animals are compared with respect to the anesthetized anim...

Cellulase from Aspergillus niger was immobilized onto β-cyclodextrin-conjugated magnetic particles by silanization and reductive amidation. The immobilized cellulase gained supermagnetism due to the magnetic nanoparticles. Ninety percent of cellulase was immobilized, but the activity of immobilized cellulase decreased by 10%. In this study, ionic liquid (1-butyl-3-methylimidazolium chloride) wa...

Globally, over 33.2 million people who mostly live in developing countries with limited access to the appropriate medical care suffer from the human immunodeficiency virus (HIV) infection. We developed an on-chip HIV capture and imaging method using quantum dots (Qdots) from fingerprick volume (10 microl) of unprocessed HIV-infected patient whole blood in anti-gp120 antibody-immobilized microfl...

We report a novel procedure to construct a normalized (equalized) cDNA library. By introduction of the highly efficient self-hybridization system between a whole mRNA population and their corresponding cDNA immobilized on latex beads, which involves relatively simple manipulations, we were able to generate an mRNA population in which the copy number of abundant species was reduced while that of...

Dermatan sulfate is a naturally occurring antithrombotic glycosaminoglycan. The antithrombin activity of several dermatan sulfate preparations has been measured in whole human plasma and found to be -55% of that in purified systems. Kinetic studies under pseudo-first-order conditions indicated that the reduction in antithrombin activity of dermatan sulfate in plasma compared with that in buffer...

Lipase enzyme producing bacteria, Pseudomonas sp., have been grown at varying oil concentrations to make it adaptive for high oil concentrations and it was found to show the maximum growth and maximum lipase activity when 40- and 30-vol% of oil respectively was used as a source of carbon in growth medium. Bacteria was immobilized with sodium alginate and used as whole cell catalyst for the tran...

We present a novel microbial whole-cell sensing system for biodefense and environmental pollution monitoring. Real time water toxicity monitoring is achieved using genetically engineered E. coli bioluminescent cells which emit light when exposed to toxic materials. The illumination intensity and its time dependence are a function of the toxicants dose and type. The bacteria are immobilized in a...

Background: Collagen, the most abundant protein in the human body, and as an extracellular matrix protein, has an important role in the fiber formation. This feature of the collagen renders  establishment of the structural skeleton in tissues. Regarding specific features associated with the collagen, such as, formation of the porous structure, permeability and hydrophilicity, it can also be use...

AlkL is an outer membrane porin from Pseudomonas. Its expression in E. coli was shown to greatly stimulate the oxidation of hydrophobic substrates by a a recombinant oxygenase enzyme system. Bacterial membranes can be perturbed for biotechnological purposes by enzymatic treatment. Some of the enzymes and their actions are discussed on this commercial site. Different chemical treatments that are...