We have identified a P450(cam) mutation, L244A, that mitigates the affinity for imidazole and substituted imidazoles while maintaining a high affinity for the natural substrate camphor. The P450(cam) L244A crystal structure solved in the absence of any ligand reveals that the I-helix is displaced inwards by over 1 A in response to the cavity created by the change from leucine to alanine. Furthe...

An imidazole moiety is often found as an integral part of fluorophores in a variety of fluorescent proteins and many such proteins display pH-dependent light emission. In contrast, synthetic fluorescent compounds with incorporated imidazoles are rare and have not been studied as pH probes. In this report, the richness of imidazole optical properties, including pH sensitivity, was demonstrated b...

Calmodulin-dependent nitric-oxide synthase from bovine brain and GH3 pituitary cells is inhibited by imidazole, 1-phenylimidazole, 2-phenylimidazole, and 4-phenylimidazole, with half-maximal inhibition occurring at 200, 25, 160, and 600 microM concentrations of inhibitor, respectively. Imidazole inhibits the maximal velocity of citrulline formation by the enzyme, but does not alter the concentr...

New, optically active ligands derived from imidazole were tested in the cyclopropanation reaction of styrene using ethyl diazoacetate and copper(I) triflate as a catalyst. DOI: https://doi.org/10.2174/157017812800221735 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-61481 Accepted Version Originally published at: Mloston, G; Mucha, P...

Molecular dynamics simulations were carried out on a concentrated system of β-d-glucopyranose and imidazole molecules in a periodic box of water at 298K. The purpose of the simulations was to determine whether or not there was any tendency for these two solutes to associate in an aqueous environment, as has previously been observed for other planar functional groups from amino acid side chains,...

: Methylated poly(l-histidine) (PLH-Me), our original polypeptide, has controlled the contents of dimethylimidazolium, τ/π-methylimidazole and imidazole groups for efficient gene delivery. The screening for the PLH-Me as DNA carrier has been carried out by use of the PLH with 25 mol% (τ-methyl, 16 mol%; π-methyl, 17 mol%; deprotonated imidazole, 41 mol%), 68 mol% (τ-methyl, 16 mol%; π-methyl, 8...

In the title compound, {[Co(3)Lu(2)(C(5)H(2)N(2)O(4))(6)(H(2)O)(4)]·2H(2)O}(n), the Lu(III) ions are seven-coordinated in a monocapped trigonal prismatic coordination geometry by six O atoms from three imidazole-4,5-dicarboxyl-ate ligands and one water O atom. The Co(II) ions are six-coordinated in a slightly distorted octa-hedral geometry and exhibit two types of coordination environments. One...

In the deoxy ferrous state of histidine-ligated heme proteins, the iron-histidine band (νFe-His) has been assigned as a stretching mode that involves a two-body motion involving the iron and histidine combined with a minor amount of heme doming. An analogous Raman band, νFe-L has been observed in the proximal cavity mutant of H93G myoglobin where the Raman band of a series of nonnative axial li...

Craig Daniels,1 Carmen Michán,2 Tino Krell,1 Amalia Roca3 and Juan L. Ramos1 Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Department of Environmental Protection, C/ Prof. Albareda, 1, E-18008 Granada, Spain. Universidad de Córdoba, Campus de Rabanales, Department of Biochemistry and Molecular Biology, Edificio Severo Ochoa C-6, 2 Planta, 14071, Córdoba, Spa...

Model search strategies play an important role in finding simultaneous susceptibility genes that are associated with a trait. More particularly, model selection via the information criteria, such as the BIC with modifications, have received considerable attention in quantitative trait loci (QTL) mapping. However, such modifications often depend upon several factors, such as sample size, prior d...