AIM The suitability of Papanicolaou staining and of hematoxylin staining for DNA single-cell cytometry was investigated in comparison to Feulgen staining. MATERIALS AND METHODS Ten normal cervical smears and ten cervical smears containing cells of a squamous cell carcinoma in situ were analyzed. The integrated optical density (IOD) of 200 epithelial cells, chosen per random, was determined us...

Quantitative analysis of histoand cytochemical components such as DNA, RNA or chromatin pattern on one hand (cytometry) and the quantitative analysis of geometric non-chemical cell and tissue components (morphometry and sterology) on the other, have developed somewhat independently. Today, many different techniques, such as morphometry, sterology, and static image and flow cytometry are well es...

Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organ...

Abstract Cellular therapies such as chimeric antigen receptor (CAR) T or NK cells undergo phenotypic characterization during discovery and development of novel therapies, biomanufacturing, upstream patient cell processing for clinical use. Samples are routinely analyzed via flow cytometry cell, B monocyte surface marker expression. Image systems perform cell-based assays that can be used a conv...

The ImageStream system combines the capabilities of microscopy and flow cytometry in a single platform for quantitative image-based cellular assays in large and heterogeneous cell populations. Here we demonstrate the simultaneous measurement of druginduced apoptosis and NF-kB nuclear translocation using a combination of intensity-based and morphometric parameters. Apoptotic and necrotic cells a...