Previously, we reported that coat protein (CP) of Wheat streak mosaic virus (WSMV) (genus Tritimovirus, family Potyviridae) tolerates deletion of amino acids 36 to 84 for efficient systemic infection of wheat. In this study, we demonstrated that WSMV mutants with deletion of CP amino acids 58 to 84 but not of 36 to 57 induced severe chlorotic streaks and spots, followed by acute chlorosis in wh...

Abstract Soybean ( Glycine max L ) is a legume rich in protein, fat, vitamins, minerals and fibre. The nutritional value of soybeans the amino acid profiles processed fermented soybean (Tempe) products need to be studied. Tempe flour from black soybean, local (Anjasmoro variety) imported (USA soybean) were analyzed proximately, including carbohydrates (spectrophotometric method), protein (Kjeld...

The objective of the present study was to develop a rapid HPLC method for simultaneous separation and quantitation of dansylated amino acids and common polyamines in the same matrix for analyzing forest tree tissues and cell cultures. The major modifications incorporated into this method as compared to previously published HPLC methods for separation of only dansyl amino acids include: use of a...

Lactation increases considerably the demand for amino acids over that required for basal needs. The demand imposed varies with the quantity of milk produced and, between species, with the protein content of milk. Using data on milk composition from Blaxter (1989) and assuming milk production of 60, 1000 and 50 000 ml/d for a rat, human subject and dairy cow respectively, the ideal protein requi...

This study was conducted to determine the effect of canning, different filling media (olive oil, corn sunflower oil and high oleic oil) storage on amino acid profile protein quality in swordfish. Glutamic acid, aspartic leucine are predominant acids raw swordfish, representing 35.62 % total acids. Thawing-salting caused significant losses methionine, glycine tyrosine. Frying moisture loss, resu...

caviar is one of the valuable and most popular fish products all over the world that are obtained from sturgeons. nowadays, the wild resources of these fishes are diminished, therefore, to meet the demand for the product, farming sturgeons has been considered. the chemical composition and the amino acids profiles of the wild and farmed roe obtained from beluga (huso huso) were compared and the ...

INTRODUCTION The aim of this study was to assess the effects of albumin dialysis on hepatic encephalopathy and circulating levels of amino acids in severe alcoholic hepatitis. METHODS The study was carried out in nine patients with severe alcoholic hepatitis and four with primary biliary cirrhosis treated with the molecular adsorbent recirculating system. Besides standard liver function tests...

Aminoacyl-tRNA synthetases (aaRSs) catalyze aminoacylation and establish the rules of genetic code. Precise manipulation of synthetase activity can alter the aminoacylation specificity to attach non-canonical amino acids to the intended transfer RNA (tRNA). Subsequently by codon-anticodon interaction between messenger RNA (mRNA) and tRNA the amino acid analogs can be assigned to specific sites ...

The profile hidden Markov model (PHMM) is widely used to assign the protein sequences to their respective families. A major limitation of a PHMM is the assumption that given states the observations (amino acids) are independent. To overcome this limitation, the dependency between amino acids in a multiple sequence alignment (MSA) which is the representative of a PHMM can be appended to the PHMM...