In recent years, emphasis has been placed on colonoscopic quality, leading to reinvestigation of basic elements of colonoscopic extubation technique, such as withdrawal time, changing patient position, operator technique, and bowel preparation [1–4], that have been shown to impact polyp and adenoma detection rates. Basic technique can be improved to maximize adenoma detection with minimal addit...

A 60-year-old man had just enjoyed a summer meal that included hamburgers, fruit, and lettuce salad. He finished it off with some ice cream, and to make it healthier he threw on it a handful of seeds from the cupboard that he understood to be flax seeds. What he had eaten instead was a handful of Datura inoxia, or Moonflower seeds (Figure 1). Within 30 minutes he felt “drugged,” and shortly the...

Abstract Introduction A 19-year-old non-verbal male with history of CHARGE syndrome, severe autism, intellectual disability, coloboma blindness OD and severely imparied vision OS, deafness, self-injurious aggressive behavior, Tetralogy Fallot status post repair, pulmonary valve replacement, hypertension, hypothyroidism, megacolon, gastrostomy tube dependence, eosinophilic esophagitis chronic ki...

agrobacterium rhizogenes causes hairy root disease in plants. these hairy roots are ge-netically stable and grow rapidly. transformed hairy roots of hyoscyamus muticus in-duced by the bacterium can produce tropane alkaloids in trace amounts of intact plant tissues. in this research, in order to compare growth and biosynthetic stability of hairy roots with wild type ones, leaf and nodal segments...

Background: The use of the desirability function approach combined with the response surface methodology (RSM), also called Desirability Optimization Methodology (DOM), has been successfully applied to solve medical, chemical, and technological questions. It is particularly effi cient for the determination of the optimal conditions in natural or industrial processes involving d...

Background: Plants infection with microorganisms as well as physical factors such as osmotic stresses especially drought stress induced particular secondary metabolite production. Objective: Plant root and shoot hyoscyamine (HYO) and scopolamine (SCO) variations were investigated under three water deficit stress (WDS) levels as 30 (W1), 60 (W2) and 90 % (W3) water depletion of field capacity a...

Black henbane (BH) or Hyoscyamus niger, has been used as a medicine since last centuries and has been described in all traditional medicines. It applies as a herbal medicine, but may induce intoxication accidentally or intentionally. All part of BH including leaves, seeds and roots contain some alkaloids such as Hyoscyamine, Atropine, Tropane and Scopolamine. BH has pharmacological effects like...

BACKGROUND Tropane alkaloids (TA) including anisodamine, anisodine, hyoscyamine and scopolamine are a group of important anticholinergic drugs with rapidly increasing market demand, so it is significant to improve TA production by biotechnological approaches. Putrescine N-methyltransferase (PMT) was considered as the first rate-limiting upstream enzyme while tropinone reductase I (TRI) was an i...