Ricin toxin A chain (RTA) binds to stalk P-proteins to reach the α-sarcin/ricin loop (SRL) where it cleaves a conserved adenine. Arginine residues at the RTA/RTB interface are involved in this interaction. To investigate the individual contribution of each arginine, we generated single, double and triple arginine mutations in RTA. The R235A mutation reduced toxicity and depurination activity mo...

After the synthesis of transmembrane peptides/proteins (TMPs), their insertion into a lipid bilayer is a fundamental biophysical process. Moreover, correct orientations of TMPs in membranes determine the normal functions they play in relevant cellular activities. In this study, we have established a method to determine the orientation of TMPs in membranes. This method is based on the use of TAM...

in this research, the interaction of hexadecyl trimahyl ammonium bromide (htab) with enzyme ureasehas been investigated comprehensively at different experimental conditions such as ionic strength, proteinconcentration using ion selective membrane electrode of surfactants. the obtained binding isotherms frompotentiometnc studies have been analyzed by different theories such as wyman binding pote...

The role of enzyme crosslinking in mediating formation of hydrophobic association in chemically denatured whey protein isolates (WPI) is examined. WPI samples denatured with dithiothreitol (DTT) and incubated at 50 8C with and without transglutaminase enzyme show dramatic differences in viscosity, with the viscosity of the sample exposed to enzyme being lower by several orders of magnitude than...

Although metal-based anticancer drugs have been recognized as the most effective agents over the organic compounds, non-selectivity and high toxic effects have limited their applications in a way that only three Pt-analogues have progressed into clinical use. These problems have spurred chemists to develop different strategies based on alternative targets. This work focuses on predicting potenc...

The effect of salts on the solvent-induced interactions between hydrophobic particles dispersed in explicit aqueous solution is investigated as a function of the salt's ionic charge density by molecular dynamics simulations. We demonstrate that aggregates of the hydrophobic particles can be formed or dissolved in response to changes in the charge density of the ions. Ions with high charge densi...

We have shown that the lattice fluid hydrogen bond ~LFHB! model can successfully quantify the first-order volume transition in hydrogels. The model predicts that a critical balance of hydrophilic and hydrophobic interactions is required for a gel to exhibit a discontinuous volume transition. In this work we will report the swelling behavior of a new thermoreversible copolymer hydrogel, which ha...

A supramolecular self-assembly has been constructed by perylene-bridged bis(β-cyclodextrin)s with water-soluble porphyrin through hydrophobic interactions, showing strong excitonic coupling interactions between perylene backbones and included porphyrins.

This paper describes inhibitors for human carbonic anhydrase II (HCAII, EC 4.2.L.L) that bind with nanomolar dissociation constants. These inhibitors were developed by exploiting interactions with hydrophobic "patches" in the lip of the active site of this enz)me. These patches are molecular surfaces presented by a phenylalanine on one face of the active-site cleft (Phe-131) and three adjacent ...

2015 Ungefär 100 kilo ATP syntetiseras i kroppen varje dag av ATP-syntas, ett sammansatt proteinkomplex med tre aktiva centra. Komplexet sitter förankrat i ett biologiskt membran med hjälp av hydrofob attraktion och består av en roterande och en statisk del. En ATP-molekyl syntetiseras per tredjedels varv. Trots att varje aktivt centrum skulle kunna fungera oberoende av de andra, sparas mycket ...