Hyaluronic acid-binding region and trypsin-link protein were prepared from bovine nasal cartilage proteoglycan complex after trypsin digestion. Binary complexes were reformed between trypsin-link protein and hyaluronic acid-binding region or hyaluronate. Upon trypsin treatment of these complexes, two fragments deriving from trypsin-link protein were characterized. One of them, of 20 kDa, corres...

ISOHE (1) HAS DESCRIBED a color reaction produced by the addition of alcoholic carbazole to uronic acids previously heated in 87% sulfuric acid. The purpose of the present report is to apply a modification of this method to the determination of hyaluronic acid in synovial fluid. The concentration of mucin in synovial fluid has been determined by the turbidity of the mucin clot (2) or by the che...

Study of Complex Nutrients, Temperature and Salts on Hyaluronic acid Production In Streptococcus zooepidermicus ATCC 43079 Nguyen Tu Hoang Khue Pham Thien Minh Vo

Cartilage proteoglycan monomers associate with hyaluronic acid to form proteoglycan aggregates. Link protein, a glycoprotein interacting with both hyaluronic acid and proteoglycan, serves to stabilize the aggregate structure. The primary structure of the link protein has been determined with a view to defining its interaction with both hyaluronic acid and proteoglycan. Thus, the link protein ha...

In this study, bi-component alginate-hyaluronic acid (AHA) fibers were developed by using two different routes. the first method, sodium alginate dope solution was extruded into a coagulation bath containing CaCl2 and subsequently dip-coated with hyaluronic (HA) whereas, in second acid-containing directly bath. The resulting AHA then dehydrated 25–100% v/v acetone solutions dried air. character...

Five distinct glycosaminoglycan fractions have been isolated from human brain of various age groups, by employing an improved fractionation procedure. Analysis of these fractions showed that human brain contains hyaluronic acid, chondroitin-4sulphate, chondroitin-6-sulphate, dermatan sulphate, heparan sulphate and two unidentified low sulphated fractions. The pattern of variation of these compo...