Factor VII has been purified up to 330,000-fold from bovine plasma by adsorption on, and elution from, barium sulfate, anion exchange chromatography, gel filtration, and affinity chromatography on benzamidine coupled to Sepharose. The purified material is inhibited by diisopropylphosphorofluoridate both in its native state and after the formation of a complex with tissue factor in the presence ...

We analyzed the mutations in patients from 10 Polish kindreds with a bleeding diathesis due to factor VII deficiency. Patients from eight families had plasma levels of factor VII coagulant activity (VII:C) and factor VII antigen (VII:Ag) that were less than 4% of normal. The coding sequence of the factor VII gene was amplified from genomic DNA by polymerase chain reaction (PCR). Sequencing demo...

Factor VII has been purified up to 330,000-fold from bovine plasma by adsorption on, and elution from, barium sulfate, anion exchange chromatography, gel filtration, and affinity chromatography on benzamidine coupled to Sepharose. The purified material is inhibited by diisopropylphosphorofluoridate both in its native state and after the formation of a complex with tissue factor in the presence ...

Factor VII levels have been measured in 100 patients with liver disease following parenteral vitamin K1 therapy. There was good agreement between specific factor VII measurements and the one-stage prothrombin time apart from six patients with compensated cirrhosis in whom the prothrombin time was prolonged despite the presence of normal factor VII levels. A mean activity of 58% was found in pat...

BACKGROUND/AIMS Low factor V and VII levels are bad prognostic indicators in fulminant hepatic failure (FHF). The prognostic importance of admission versus follow up levels of these factors in patients with acute hepatitis and coagulopathy without encephalopathy has not been evaluated. METHODS Clinical and laboratory data from 68 consecutive patients with acute hepatitis and coagulopathy but ...

Vienna, Austria) as a quick reversal agent in emergency situations. Levy et al. cited the study by Marlu et al.2 but made no mention of the results on FEIBA as a reversal agent reported in that study and chose only to discuss the results on prothrombin complex concentrate and recombinant factor VIIa, while acknowledging that there is an activated form of 4-factor prothrombin complex concentrate...