Oxygen is a key modulator of cellular pathways, but current techniques for probing this important variable lack precise control and efficiency. A microfabricated polydimethylsiloxane (PDMS) insert for multiwell plates has been developed to provide spatial and temporal control over the gas concentration in each well independent of the global incubator condition. Human dermal fibroblast adult cel...

Adipose stem cell-conditioned medium may promote human dermal fibroblast (HDF) proliferation and migration by activating paracrine peptides during the re-epithelization phase of wound healing. Human antimicrobial peptide LL-37 is upregulated in the skin epithelium as part of the normal response to injury. The effects of conditioned medium (CM) from LL-37 treated adipose stem cells (ASCs) on cut...

the interaction of herpes simplex virus type 1 (hsv-1) with human fetal foreskin fibroblast (hfff) cell was studied using a recent isolate of hsv-1 which was propagated in hep-2 cells. hfff cells were challenged with hsv-1 with a multiplicity of infection (moi) of 1 virus/cell for 24 hours. flow cytometric analysis demonstrated that hsv-1 challenged hfff cells expressed increased levels of α2β1...

BACKGROUND Platelet gel is a source of many soluble proteins that can modulate tissue regeneration. Dermal fibroblasts play an important role in tissue remodeling and wound healing. OBJECTIVE The study was performed to investigate the influence of platelet gel and its active ingredients on the proliferation of human dermal fibroblasts in vitro. METHODS A fibroblast culture was established f...

The interaction of Herpes Simplex Virus type 1 (HSV-1) with human fetal foreskin fibroblast (HFFF) cell was studied using a recent isolate of HSV-1 which was propagated in Hep-2 cells. HFFF cells were challenged with HSV-1 with a multiplicity of infection (MOI) of 1 virus/cell for 24 hours. Flow cytometric analysis demonstrated that HSV-1 challenged HFFF cells expressed increased levels of α2β1...

Human activated T cells adhere to synovial fibroblast-like cells in vitro. The present study was conducted to investigate the consequences of T cell-synovial fibroblast interactions with regard to induction of adhesion molecules and proinflammatory cytokines. A sensitive Western blot technique, polymerase chain reaction (PCR) amplification, and fluorescence-activated cell sorter (FACS) analysis...

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Chronic inflammation is associated with an infiltration of mononuclear cells, fibroblast proliferation, and elevated levels of prostaglandin (PG) E2. Mononuclear cell conditioned factor (MNCF) medium (5%) stimulated a 100-fold increase in basal human dermal fibroblast PGE2 release over 48 h as compared with fibroblasts that were incubated with control medium (conditioned medium prepared without...

Recessive dystrophic epidermolysis bullosa (RDEB) is a devastating progressive skin fragility disorder caused by deficiency in type VII collagen (C7) and favored pro-fibrotic pro-tumorigenic dermal stroma. RDEB has served as paradigmatic model of injury-driven fibrosis. Nevertheless, the contribution epithelial cells to fibrosis been scarcely addressed progression. To study effect keratinocytes...