Time-resolved Fourier transform infrared (FTIR) difference spectra of the halorhodopsin (hR) photocycle have been collected from 3 micros to 100 ms in saturating concentrations of KCl or KBr. Kinetic analysis of these data revealed two decay processes, with time constants of tau(1) approximately 150 micros and tau(2) approximately 16 ms in the presence of either halide, with tau(2) describing t...

AIMS The objective of this study was to assess in vitro, whether heat-killed (HK) lactic acid bacteria cells and fractionations of HK cells could suppress the viability of human cancer cells and inhibit the cytotoxicity associated with oxidative stress. METHODS AND RESULTS Among the strains, the HK cells of Lactobacillus acidophilus 606 and Lactobacillus casei ATCC 393 exhibited the most prof...

BACKGROUND Creatine kinase (CK) and hexokinase (HK) play a key role in myocardial energy homeostasis. We aimed to determine CK and HK expression and enzyme activity in the left (LV) and right (RV) ventricles of rats adapted for 3 weeks to normobaric hypoxia (10 % O2) either continuously (CNH) or intermittently with 1-h or 16-h normoxic episode per day. METHODS The Real-Time RT-PCR, Western bl...

Impairment of lung aconitase activity, citric acid cycle, and mitochondrial respiration by hyperoxia necessitates the elevation of glycolysis for energy production and of pentose shunt activity for reducing equivalents. The molecular mechanisms that allow increased glucose utilization are unknown. Adult male and female rats were adapted to sublethal hyperoxia, equivalent to 83% oxygen at sea le...

The H(+)/K(+)-ATPase alpha2 subunit (HK alpha 2) of distal colon and renal collecting ducts plays a critical role in potassium and acid-base homeostasis. The isolation and complete sequence of the murine HK alpha 2 gene are reported. The HK alpha 2 gene contains 23 exons and spans 23.5 kb of genomic DNA. The exon/intron organization is comparable to that of the human ATP1AL1 gene. Primer extens...

A comparative study of the Na+-stimulated adenosine triphosphatase system in high potassium (HK) and low potassium (LK) sheep red cell membranes was carried out. For HK:LK activity, the approximate ratios were as follows: 10 for Na+-ATPase, 13 for Na+, K+-ATPase, and 2.7 for [%+ ADP-ATP exchange activity; the ratio of Na+-stimulated membrane phosphorylation was approximately 7. With Na+ present...

A unique cDNA for hexokinase (HK) was identified from the observation that HKR activity is predominant in reticulopoly(A) RNA of human reticulocytes by anchored polymercytes. Northern blot analysis showed that it was expressed ase chain reaction. This appeared to represent the cDNA for in the reticulocytes and in the K562 erythroleukemic cell line, the red blood cell (RBC)–specific HK isozyme (...

Candida tropicalis is one of the most frequent causes of serious disseminated candidiasis in human patients infected by non-albicans Candida species, but still relatively little is known about its virulence mechanisms. In our current study, the interactions between the cell surface of this species and a multifunctional human protein - high-molecular-mass kininogen (HK), an important component o...

Fusobacterium necrophorum causes Lemièrre's syndrome, a serious disease with septic thrombophlebitis of the internal jugular vein, pulmonary involvement, and systemic inflammation. The contact system is a link between inflammation and coagulation, and contact activation by the bacteria could therefore contribute to the abnormal coagulation and inflammation seen in patients with Lemièrre's syndr...

Hexokinases (HKs) are the enzymes that catalyses the ATP dependent phosphorylation of Hexose sugars to Hexose-6-Phosphate (Hex-6-P). There exist four different forms of HKs namely HK-I, HK-II, HK-III and HK-IV and all of them share a common ATP binding site core surrounded by more variable sequence that determine substrate affinities. Although they share a common binding site but they differ in...