Protected deoxyribonucleotides were identified by field desorption mass spectrometry in eluate fractions from high performance liquid chromatography. The production of abundant cationized molecules and the formation of structural significant fragments by thermally/field induced processes allowed a direct and unambiguous identification of the synthesized products. In addition, indications can be...

A method was developed for the simultaneous determination of urinary vanilmandelic acid and homovanillic acid, which included a two step prepurification and a reversed-phase high-performance liquid chromatography with amperometric detection. Conditions were evaluated for performing measurements with the amperometric detector free from electric interference. The method was linear between 2.5 and...

The increased separation efficiency provided by the new technology of column packed with core-shell particles in high-performance liquid chromatography (HPLC) has resulted in their widespread diffusion in several analytical fields: from pharmaceutical, biological, environmental, and toxicological. The present paper presents their most recent applications in food analysis. Their use has proved t...

We have developed a simplified and improved high performance liquid chromatography (HPLC) method for the detection and quantitation of tissue lipid using a new laser light-scattering detector (Varex model ELSD II). This detector has a limit of sensitivity of 50 ng for neutral lipid and 200 ng for most phospholipids with excellent reproducibility. By coupling the ELSD II with a ternary gradient ...

Pyrraline is one of the advanced glycation end products formed under non-enzymatic and non-oxidative conditions in vivo. In this study, we developed a novel method for determination of urinary pyrraline using solid-phase extraction as a pretreatment procedure prior to determination by high performance liquid chromatography (HPLC). The Oasis HLB solid-phase extraction cartridge was used for pret...

The chemical composition of grape berries is influenced by various environmental conditions often considered to be representative of a "terroir". If grapes from a given terroir are assumed to reflect this origin in their chemical compositions, the corresponding wine should also reflect it. The aim of this work was therefore to reveal the "terroir" expression within the chemodiversity of grapes ...

Interest in metal complexes in modern inorganic chemistry has resulted in increasing demands for the analysis of these compounds. This paper reviews the most recent applications of high-performance liquid chromatography (HPLC) to the analysis of metal complexes. The review centres on the use of the technique in metal complex syntheses, reactions, characterizations and complexations and retentio...

In the present study we used chiral high performance liquid chromatography and gas chromatography to determine the stereoisomers of racemic alpha-tocopherol. The Chiralcel OD-H and Silar-100 AT columns were used. Statistical evaluation of the results established the precision of these methods.

In this investigation, sensitive and reproducible methods are described for quantitative determination of deflazacort in the presence of its degradation product. The method was based on high performance liquid chromatography of the drug from its degradation product on reverse phase using Acquity UPLC BEH C18 columns (1.7 µm, 2.1 mm × 150 mm) using acetonitrile and water (40:60 V/V) at a flow ra...

We describe a reversed-phase HPLC method for determination of total mycophenolic acid (MPA), its free concentration (MPAf), and the glucuronide metabolite (MPAG), based on simple sample preparation and gradient elution chromatography. The compounds were quantified in parallel by absorbance at 254 nm and 215 nm in the internal standard mode. Linearity was verified up to 50 mg/L for MPA and up to...